转基因水稻外源基因的遗传和表达初步研究

将天蚕抗菌肽B基因应用基因枪法导入水稻TN1发芽肿胚，获得转基因植株2株，转基因水稻T1－T5 5个世代不同株系，对bar基因、抗菌肽基因的遗传和表达进行初步研究。结果表明：转基因后代株系bar^R:bar^S在部分符合3：1分离比率。T1、T4转基因株系PCR、Southern印迹分析表明，T18株有抗菌肽B基因；T4仍有4个株系有抗菌肽基因，其他的株系基因丢失，T3 1个株系Northern印迹证明抗菌肽基因在RNA水平有表达；抗菌肽B转基因水委对白叶枯病的抗病性有提高，抗性能传递到高世代，在T3得到抗病性提高的株系。

Primary Studies on Inheritance and Expression of Foreign Gene in Transgenic Rice

Two transgenic plants transformed with cecropin B gene usingbiolistic method were generated from germinative embryo of rice variety TN1. The inheritance and expression of bar and cecropin B gene in different transgenic plantlines of TN1 from 5 generation of T1 to T5 were primary studied. Results showed that the segregation ratio of barR:barS was 3∶1. The results of PCR and Southern blot analysis indicated that eight of T1 and four of T4 transgenic lines had cecropin B gene，the other plantlines of T4 showed lost of cecropin gene. One of T3 transgenic line expressed at mRNA level by Northern blot. The resistance to bacterial blight delivered from cecropin B transgene could be segregated and transmitted to high generation, resistance- improved plantlines were obtained in T3 generation.