| 鸡毒支原体DNA提取和纯化的研究 |
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| 引用本文： | 郭建华,陈德威.鸡毒支原体DNA提取和纯化的研究[J].山西农业大学学报(自然科学版),1998,18(2):110-111. |
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| 作者姓名： | 郭建华 陈德威 |
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| 作者单位： | 山西农业大学动物医学系(郭建华)，中国农业大学实验动物所(陈德威)，天津农学院动物科技学系(杨百亮)，山西省畜牧兽医学校(赵良仓) |
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| 摘 要： | 本文报道了鸡毒支原体ＤＮＡ提取和纯化过程。应用不同方法自鸡毒支原体培养物中共提取１０批鸡毒支原体ＤＮＡ。ＤＮＡ片段大小均一，在琼脂糖凝胶电泳中呈一条带，ＤＮＡ溶液的ＯＤ２６０／ＯＤ２８０接近１．８。用限制性核酸内场酶ＢａｍＨＩ切割，呈清晰的ＤＮＡ条带。
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| 关 键 词： | 鸡毒支原体 琼脂糖凝胶电泳 酶切图谱 |
Study on Extraction and Purification of Mycopeasma Gaeeisepticum DNA |
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| Guo jianhua.Study on Extraction and Purification of Mycopeasma Gaeeisepticum DNA[J].Journal of Shanxi Agricultural University,1998,18(2):110-111. |
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| Authors: | Guo jianhua |
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| Abstract: | The extraction and purification of Mycoplasma galliseptium DNA with different methods were described.DNA electrophored in horizontal gels containing 0 6% agarose showed a major band.BamHI digested MG DNA present several fragments.The OD260nm/OD280nm of DNA solution was nearly 1 8. |
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| Keywords: | Mycoplasma galliseptium agarose gel electrophoresis Restrietion Eneyine Cutting Pattern |
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