| 草菇S-腺苷-L-高半胱氨酸水解酶基因上游序列的克隆和分析 |
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| 引用本文： | 孙晓红,陈明杰,汪虹,冯爱萍,潘迎捷. 草菇S-腺苷-L-高半胱氨酸水解酶基因上游序列的克隆和分析[J]. 食用菌学报, 2010, 17(3) |
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| 作者姓名： | 孙晓红 陈明杰 汪虹 冯爱萍 潘迎捷 |
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| 基金项目： | 农业部公益行业科研专项 |
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| 摘 要： | 根据草菇(Volvariella volvacea)S-腺苷-L-高半胱氨酸水解酶基因(sahh)全长序列设计两个嵌套引物,与Takara LA PCR in vitro cloning试剂盒的接头引物结合进行巢式PCR扩增,得到sahh上游调控序列,通过序列分析发现,该序列含有1个TATA盒和4个CAAT盒.应用MatInspector程序分析sahh上游调控序列,发现该区域存在1个耐盐因子,1个热激因子,2个DRE元件和2个ABA应答元件.
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| 关 键 词： | S-腺苷-L-高半胱氨酸水解酶基因 调控序列 草菇 |
Cloning and Sequence Analysis of an Upstream Sequence of a S-Adenosyl-L-homocysteine Hydrolase Gene from Volvariella volvacea |
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| SUN Xiaohong,CHEN Mingjie,WANG Hong,FENG Aiping,PAN Yingjie. Cloning and Sequence Analysis of an Upstream Sequence of a S-Adenosyl-L-homocysteine Hydrolase Gene from Volvariella volvacea[J]. Acta Edulis Fungi, 2010, 17(3) |
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| Authors: | SUN Xiaohong CHEN Mingjie WANG Hong FENG Aiping PAN Yingjie |
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