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| 西洋参发根的诱导及其培养条件的研究 | |
| 引用本文： | 贾冬梅,赵寿经,钱延春,吴颖,杨柏明,张博. 西洋参发根的诱导及其培养条件的研究[J]. 吉林农业大学学报, 2010, 32(1): 58-61 |
| 作者姓名： | 贾冬梅 赵寿经 钱延春 吴颖 杨柏明 张博 |
| 作者单位： | 1. 吉林大学植物科学学院,长春,130062 2. 吉林大学生物与农业工程学院,长春,130022 3. 吉林大学生命科学学院,长春,130000 |
| 基金项目： | 吉林省科技发展计划项目(20060546);;吉林大学农学部青年基金项目(200826) |
| 摘 要： | 利用发根农杆菌A4菌株在西洋参根外植体上直接诱导产生发根。在1/2 MS和MS固体培养基上得到西洋参发根,并在B5液体培养基上建立起发根离体培养系,经连续多代的培养,发根仍保持旺盛生长状态。PCR扩增结果表明,发根农杆菌Ri质粒的rolC基因已整合到西洋参发根基因组中并得到表达。利用紫外分光光度法测得西洋参发根的总皂苷含量达3.88%。经对发根比生长速率的测定,确定1/2 B5培养基(15 g/L蔗糖)、摇床转速100 r/min、每4周更换1次培养基并继代培养1次为西洋参发根生长适宜条件。 |
| 关 键 词： | 生物工程 发根 农杆菌 西洋参 |
| 收稿时间： | 2008-12-29 |
| 修稿时间： | 2009-05-15 |
Induction of Hairy Roots of Panax quinquefolium by Ri Plasmid and Research on Suitable Culture Condition of Panax quinquefolium Hairy Roots |
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| JIA Dong-mei,ZHAO Shou-jing,QIAN Yan-chun,WU Ying,YANG Bai-ming,ZHANG Bo. Induction of Hairy Roots of Panax quinquefolium by Ri Plasmid and Research on Suitable Culture Condition of Panax quinquefolium Hairy Roots[J]. Journal of Jilin Agricultural University, 2010, 32(1): 58-61 | |
| Authors: | JIA Dong-mei ZHAO Shou-jing QIAN Yan-chun WU Ying YANG Bai-ming ZHANG Bo |
| Affiliation: | 1.College of Plant Science, Jilin University, Changchun 130062, China|2. College of Biology and Agriculture Engineering, Jilin University, Changchun 130022, China|3.College of Life Science, Jilin University, Changchun 130000, China |
| Abstract: | Hairy roots of Panax quinquefolium L.were induced from the root explants of two-year old Panax quinquefolium L.by Agrobacterium rhizogenes A4 with direct inoculation,the cultured clones of the hairy roots were established on solid 1/2 MS and MS mediums.After several times of subcultures,the hairy roots still maintained vigorous growth.After the determination of UV-spectrophotometry,the total ginsenosides content in the hairy root came up to 3.88%.The suitable culture conditions for Panax quinquefolium L.hai... |
| Keywords: | bioengineering hairy root Agrobacterium rhizogenes Panax quinquefolium L. |
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