Diversity of Midgut Microbial Community of Chilo suppressalis Exposed to Chlorobenzamide

【Objective】We aim to illuminate the structural changes in the midgut microbial community of Chilo suppressalis exposed to chlorobenzamide.【Method】Macrogenomic sequencing and in vitro culture were used to analyze the diversity of midgut microbiota of Chilo suppressalis treated with different concentrations (100, 200, 400 µg/mL) of chlorobenzamide .【Result】The results showed that chlorobenzamide treatment decreased the abundance and diversity of the midgut bacteria of Chilo suppressalis,and the OTU number and specific OTU number of the intestinal bacteria of treated population were lower than those of the control; the proportion of Morganella, Provincia and Proteus in the chlorobenzamide treated population increased significantly. Meanwhile, the result of in vitro culture indicated besides enterobacter, Lelliotti nimipressuralis, Leclercia decarboxylata, Pantoea agglomerans were isolated from chlorobenzamide treated Chilo suppressalis population. COG classification and KEGG analysis didn’t show significant difference in number and relative abundance of functional genes among control and three chlorobenzamide treated populations.【Conclusion】The study had clarified the diversity of intestinal bacteria and the relationship between gut bacteria of Chilo suppressalis and the formation of chlorobenzamide resistance, and will lay a scientific basis for the role of intestinal bacteria in resistance of Chilo suppressalis to chlorobenzamide.