马铃薯高效染色体加倍方法建立与抗寒资源创制 |
| |
基金项目: | This study was supported by the China Agriculture Research System(CARS-09-P07);and the National Natural Science Foundation of China(31871685) |
| |
摘 要: |
|
收稿时间: | 2020-03-19 |
Establishment of a high efficient method for chromosome doubling and exploration of cold-resistant resources in potato |
| |
Authors: | DONG Jian-Ke TU Wei WANG Hai-Bo YING Jing-Wen DU Juan ZHAO Xi-Juan ZHAO Qing-Hao HUANG Wei CAI Xing-Kui SONG Bo-Tao |
| |
Institution: | College of Horticulture and Forestry Sciences, Huazhong Agricultural University / Key Laboratory of Horticultural Plant Biology (HZAU), Ministry of Education / Key Laboratory of Potato Biology and Biotechnology, Ministry of Agriculture and Rural Affairs, Wuhan 430070, Hubei, China |
| |
Abstract: | In this work, different treatment combinations including colchicine concentrations and treatment methods as well as different time points was used to investigate the survival rate and chromosome doubling efficiency of potato tissue culture plantlets. The potato chromosome doubling by using colchicine had been successfully optimized. The potato plantlet stems treated with 0.1% colchicine for three days shaking at 120 r min-1 showed the highest doubling efficiency due to its better contact to the colchicine solution. Compared with other potato chromosome doubling techniques, this method has much higher chromosome doubling rate, shorter time treatment and easier to operate, so that it could provide a higher efficient method for potato ploidy operation. In the meantime, compared with the diploid, the tetraploid interspecific hybrids showed differences in the morphological characteristic, which had higher plants, thicker stem, bigger petals and pollen grain. In addition, no significant difference was found between diploid and tetraploid interspecific hybrids in terms of cold resistance, but both significantly enhanced cold resistance compared with the common potato cultivar. Taken together, the doubled interspecific hybrids could sever for improving cold resistance of potato cultivars. |
| |
Keywords: | potato colchicine chromosome doubling cold resistance |
|
| 点击此处可从《作物学报》浏览原始摘要信息 |
| 点击此处可从《作物学报》下载免费的PDF全文 |
|