Development and Utilization of Functional Markers for Imidazolinone Herbicides Resistance Gene in japonica Rice Variety Jinjing 818 |
| |
Authors: | WANG Guangda GAO Peng YANG Wenyan CUI Ao ZHAO Jianhua FENG Zhiming CAO Wenlei CHEN Zongxiang ZUO Shimin |
| |
Institution: | 1. Jiangsu Key Laboratory of Crop Genetics and Physiology / Key Laboratory of Plant Functional Genomics of Ministry of Education / Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, College of Agriculture, Yangzhou University, Yangzhou 225009, China;2.Jiangsu Collaborative Innovation Center for Modern Industrial Technology of Grain Crops, Yangzhou University, Yangzhou 225009, China |
| |
Abstract: | 【Objective】Cultivating herbicide resistant rice varieties is an important direction in rice breeding. Developing efficient functional markers can accelerate the screening of herbicide resistance trait. 【Method】dCAPS and KASP techniques were used to analyze the genotypes of the experimental materials. In addition, the phenotypes were identified by herbicide spraying test. 【Result】Through sequence alignment analysis, we further confirmed that the mutation of 627 amino acid of acetolactate synthase (ALS) gene from serine (S) into asparagine (N) in Jinjing 818 (here after named ALS627N) produces strong resistance to imidazolinone herbicides. According to the DNA sequence flanking the mutation site, we developed two novel functional markers, dC-ALS-627 and K-ALS-627, for detecting this resistant allele ALS627N. The two markers can effectively distinguish homozygous ALS627N type, heterozygous type and homozygous wild type. Combined with herbicide spraying test, we confirmed that ALS627N is a dominant herbicide resistant gene. 【Conclusion】 We validated that the two markers have great potential in marker-assisted breeding for ALS627N gene. Totally, this study will accelerate the development of new varieties with resistance to imidazolinone herbicides, and have significance in breeding practice. |
| |
Keywords: | rice imidazolinone herbicide acetolactate synthase functional marker assisted selection |
|
| 点击此处可从《中国水稻科学》浏览原始摘要信息 |
| 点击此处可从《中国水稻科学》下载免费的PDF全文 |
|