我国6个省份番茄褪绿病毒和番茄黄化曲叶病毒复合侵染率及序列系统发育分析

于2017～2019年在我国6个省份的番茄主产区，采集疑似番茄褪绿病毒（tomato chlorosis virus，ToCV）和（或）番茄黄化曲叶病毒（tomato yellow leaf curl virus，TYLCV）侵染的番茄样本，利用特异性引物进行RT-PCR 或PCR 扩增，将符合预期条带大小的PCR 产物测序后进行系统发育分析。结果表明，我国6 个省份番茄ToCV 和TYLCV 的复合侵染率为25.29%，其中山东省两种病毒复合侵染率最高，为46.43%。序列分析结果表明，测定的ToCV CP 基因序列与已报道的对应序列同源性在98.30% 以上，ToCV CP 基因序列没有发生明显的遗传分化；TYLCV 基因组部分序列与已报道的对应序列同源性在96.00% 以上，没有发生明显的遗传分化。

Analysis on ToCV and TYLCV Co-infection Ratio and Sequence Phylogeny in 6 Provinces of China

From 2017-2019，in major tomato producing areas of 6 Chinese provinces，tomato samples suspected to be infected by tomato chlorosis virus（ToCV） and（or） tomato yellow leaf curl virus（TYLCV）were collected．This paper conducted RT-PCR or PCR by specific primers，then carried out phylogenetic analysis on PCR products conforming to expected band size after sequencing．Results showed that the recombination co-infection ratio of ToCV and TYLCV from 6 Chinese provinces was 25.29%，among them those of 2 virus from Shandong Province was the highest-46.43%．Results of sequences analysis indicated that the homology of measured ToCV CP gene sequence with reported corresponding sequence was over 98.30%．There were no obvious genetic differentiation in ToCV CP gene sequences．The homology of partial sequence of TYLCV genome with published corresponding sequence was over 96.00%，and no distinct genetic differentiation was occurred．