| 高产纤维素酶木霉菌株的筛选 |
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| 引用本文： | 檀华龙,袁明维,伊兴领,王岩岩,马松.高产纤维素酶木霉菌株的筛选[J].安徽农学通报,2010,16(23):51-53,56. |
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| 作者姓名： | 檀华龙 袁明维 伊兴领 王岩岩 马松 |
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| 作者单位： | 安徽科技学院生命科学学院,安徽凤阳,233100;安徽科技学院生命科学学院,安徽凤阳,233100;安徽科技学院生命科学学院,安徽凤阳,233100;安徽科技学院生命科学学院,安徽凤阳,233100;安徽科技学院生命科学学院,安徽凤阳,233100 |
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| 基金项目： | 安徽科技学院大学生创新课题项目 |
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| 摘 要： | 从18株木霉中筛选出产纤维素酶能力较强的菌株。将18株菌株活化处理后接种于液体发酵培养基上，在30℃，转速为180r／min条件下摇床培养7d，抽滤，得粗制酶液。通过对滤纸酶活力测定的反应中观察，菌株H4、F1、C11、H2、BI、05、A13分解滤纸务效果较好，然后分别测此7株木霉的羧甲基纤维素酶、滤纸酶、β-葡萄糖苷酶活力，综合比较后，筛选出H4、F1和C113株产纤维素酶能力较高的菌株。
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| 关 键 词： | 纤维素酶 羧甲基纤维素酶 滤纸酶 β-葡萄糖苷酶 木霉 |
Screening of High-yield Cellulase Trichoderma |
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| Institution: | Tan Hualong et al. (College of Life Sciences, Anhui Science and Technology University, Fengyang 233100, China) |
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| Abstract: | The high - producing cellulase strains were screened from the 18 strains of Trichoderma saved the laboratory. These 18 strains were inoculated in the liquid fermentation medium and cultured for 7 days at 30℃ under rotate speed of 180rpm. The crude enzyme solution was obtained by filtration. The strains H4, F1, Cll, H2, B1, 05 and A13 were screened by the reaction of filter paper decompostion. After activity determination of Carboxymethyl Cellulose, Filter paper enzyme and β - glucosidase, the strains H4, FI and C11 were selected according to their high cellulose production capacity. |
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| Keywords: | Cellulose Carboxymethyl Cellulose Filter paper enzyme β - glueosidase activity Trichoderma |
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