| 北部湾海绵（Siphonochalina flexa）共附生细菌多样性及抑甘蔗鞭黑粉菌活性研究 |
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| 引用本文： | 江蕾,李蜜,李智,易湘茜,高程海,周桂. 北部湾海绵（Siphonochalina flexa）共附生细菌多样性及抑甘蔗鞭黑粉菌活性研究[J]. 热带作物学报, 2019, 40(7): 1421-1427. DOI: 10.3969/j.issn.1000-2561.2019.07.025 |
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| 作者姓名： | 江蕾 李蜜 李智 易湘茜 高程海 周桂 |
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| 作者单位： | 1. 广西中医药大学海洋药物研究院/药学院,广西南宁 5302002. 广西民族大学海洋与生物技术学院/广西多糖材料与改性重点实验室培育基地/广西高校微生物与植物资源利用重点实验室,广西南宁 530006 |
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| 基金项目： | 国家自然科学基金项目(No.41566004);广西自然科学基金项目(No.2016GXNSFBA380095);广西中医药大学岐黄工程高层次人才团队培育项目(No.2018006) |
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| 摘 要： | 分离纯化广西怪石滩海域海绵可培养共附生细菌,分析其多样性并研究共附生细菌发酵产物对甘蔗鞭黑粉菌的抑制活性,为研发新型生物农药提供参考依据。采用8种培养基分离纯化海绵共附生细菌,运用16S rDNA测序方法进行菌种鉴定及多样性分析,采用牛津杯法测试细菌发酵产物对甘蔗鞭黑粉菌的抑制效果。经形态学分析及16S rDNA比对,海绵中共获得34株共附生细菌,隶属于18科24属。活性检测结果表示,存在14株对甘蔗鞭黑粉菌有抑制效果的菌株。海绵中可培养共附生细菌物种多样性丰富,部分菌株的发酵产物对甘蔗鞭黑粉菌具有较强的抑制效果,有成为新型生物农药的潜力。
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| 关 键 词： | 海绵 共附生细菌 物种多样性 甘蔗鞭黑粉菌 抑菌活性 |
| 收稿时间： | 2018-07-07 |
Diversity and Antifungal Activity Against Sporisorium scitamineum of Bacteria Isolated from Marine Sponge Siphonochalina flexa from Beibu Gulf |
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| JIANG Lei,LI Mi,LI Zhi,YI Xiangxi,GAO Chenghai,ZHOU Gui. Diversity and Antifungal Activity Against Sporisorium scitamineum of Bacteria Isolated from Marine Sponge Siphonochalina flexa from Beibu Gulf[J]. Chinese Journal of Tropical Crops, 2019, 40(7): 1421-1427. DOI: 10.3969/j.issn.1000-2561.2019.07.025 |
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| Authors: | JIANG Lei LI Mi LI Zhi YI Xiangxi GAO Chenghai ZHOU Gui |
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| Affiliation: | 1. Institute of Marine Medicine / Faculty of Pharmacy, Guangxi University of Chinese Medicine, Nanning, Guangxi 530200, China2. School of Marine Sciences and Biotechnology, Guangxi University of Nationalities / Guangxi Key Laboratory Cultivation Base for Polysaccharide Materials and Modifications / Guangxi Colleges and Universities Key Laboratory of Utilization of Microbial and Botanical Resources, Nanning, Guangxi 530006, China |
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| Abstract: | The purpose of this study was to investigate the distribution and the diversity of co-bacteria of Siphonochalina flexa isolated from Guaishi sea beach and to study its antifungal activities of the fermentation liquids against Sporisorium scitamineum. Eight different isolation media were used to isolate and purify the co-bacteria from S. flexa. The 16S rDNA was employed to explore the diversity of the isolated strains. The effects of the fermentation liqiuds against S. scitamineum were tested with the method of Oxford cup. After the morphological analysis and 16S rDNA alignment, 34 strains of co-bacteria were isolated from S. flexa, belonging to 18 families and 24 genera. Through testing the antifungal activities, we screened 14 kinds of bacteria that had the inhibitory effect on S. scitamineum. The cultivated bacteria in S. flexa were rich in species and most of them showed strong activity of against S. scitamineum. |
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| Keywords: | Siphonochalina flexa co-bacteria species diversity Sporisorium scitamineum antifungal activities |
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