两亲性壳聚糖/槲皮素纳米胶束的制备及其理化性质研究

槲皮素(QCT)是一种类黄酮化合物,具有较强的抗氧化、抗炎等作用,在热带植物界中广泛存在。但由于槲皮素溶解度低、稳定性差,因此限制了其在食品、医药中的应用。本研究以脱氧胆酸(DA)为疏水基团,N-乙酰-L-半胱氨酸(NAC)作为亲水基团合成了一种新型两亲性壳聚糖(ACS),并制备了两亲性壳聚糖槲皮素纳米胶束(ACS-QNMs)。结果表明,傅里叶红外光谱分析证明DA和NAC已成功接枝到壳聚糖上,荧光分光光度计测定空白胶束的临界胶束浓度值(CMC)为26.92μg/mL,扫描电镜观察纳米载药胶束外观呈球形且分布均匀。纳米粒度电位分析仪测定纳米胶束电位为31 mV。研究表明,槲皮素经两性壳聚糖包埋后,能在人体温度37℃下缓慢释放,同时在室温下稳定储存。

Preparation and Physicochemical Properties of Amphiphilic Chitosan/ Quercetin nanomicelles

Quercetin (QCT) is a flavonoid compound with strong antioxidant and anti-inflammatory effects, which is widely found in tropical plants. However, the low solubility and poor stability of quercetin limit its application in food and medicine. In this study, a novel amphiphilic chitosan (ACS) with deoxycholic acid (DA) as the hydrophobic group and n-acetyl-l-cysteine (NAC) as the hydrophilic group was synthesized, and the amphiphilic chitosan quercetin nanoparticle micelle (ACS-QNMs) was prepared and characterized. FTIR showed that DA and NAC were grafted onto chitosan successfully. The critical micelle concentration (CMC) of blank micelles was 26.92 μg/mL. Scanning electron microscopy (SEM) was used to observe the spherical appearance and uniform distribution of nanoparticles. The micelle potential was determined by nano particle size potentiometer to be 31 mV. The results showed that quercetin could be released in vivo and stored stably at room temperature after being embedded in nanomicelles.