HSP70及HSP70 mRNA在热应激肉鸡组织中的定位

利用间接法免疫组织化学技术和RNA探针原位杂交技术，研究了HSP70 及HSP70 mRNA在热应激6 h处理肉鸡心脏、肝脏、肺脏、肾脏、脾脏、胸腺和法氏囊等组织中的分布。结果显示，热应激6 h时HSP70 mRNA主要分布在肉鸡肝脏和肺脏中的血管壁及其周围，心肌纤维也有少量阳性信号，而脾脏、法氏囊和胸腺等组织未检出现阳性信号；热应激6 h时HSP70主要分布在所检肉鸡各种组织的血管壁。提示HSP70与热应激肉鸡心血管系统具有相关性，可能与心血管系统的功能和损伤有关。

Localizations of HSP70 and HSP70 mRNA in the Tissues of Heat Stressed Broilers

The localizations of heat shock protein 70 (HSP70) and HSP70 mRNA in heart,liver,lung,kidney,spleen,thymus and bursa of Fabricius of 6 h heat stressed broilers were studied by immunohistochemistry and in situ hybridization. The result showed that the positive signals of HSP70 mRNA at 6 h heat stress were localized in the liver and lung,especially in the wall of vessel. The weak positive signals were seen in the myocardial cells. No significant signals were observed in spleen,thymus and bursa of Fabricius;HSP70 signals were localized in the wall of vessel of all detected tissues at 6 h heat stressed broilers. The localizations of HSP70 and HSP70 mRNA suggest that HSP70 is correlation with the cardiovascular function.