花叶绿萝的多倍体诱导及快速繁殖

为改良现有的绿萝栽培品种,丰富遗传育种资源。在无菌条件下,将丛生芽浸入0.05%,0.1%,0.2%,0.5%秋水仙素水溶液中1d,然后转入MS 3mg·L$C16-BA 0.2mg·L$C1NAA中培养。培养30d后,4种处理都诱导出了变异芽,通过根尖细胞染色体检测,变异芽为四倍体或嵌合体,其中以浓度为0.2%的秋水仙素溶液浸泡处理效果最好,得到的变异芽71%为纯合的四倍体,其染色体数量由58(2n=2x=58)变为116(2n=4x=116)。

RAPID PROPAGATION AND POLYPLOIDY INDUCTION IN SCINDAPSUS AURUS CV.WILCOXII

Under aseptic conditions, caespitose shoots of Scindapsus aurus were dipped in 0.05%,0.1%,0.2% and 0.5% colchicine solutions for 1 day and then transferred to MS 6-BA 3 mg/L NAA 0.5 mg/L. Thirty days later shoots were successfully induced in all the four treatments. Examination of the chromosomes of the root tip cells of the regenerated plantlets revealed that they were either tetraploids or chimeras. Treatment with colchicine solution at 0.2% for 1 day gave the best results and 75% of the variant buds obtained were tetraploids with a chromosome number of 116 (2n=4x=116) as compared to the diploid counterparts (2n=2x=58).