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| 利用ISSR标记技术鉴定甜玉米种子纯度的研究 | |
| 引用本文： | 陈兆贵,江新晓,曾秀文,张涛,黄俏婷. 利用ISSR标记技术鉴定甜玉米种子纯度的研究[J]. 玉米科学, 2010, 18(1): 46-48 |
| 作者姓名： | 陈兆贵 江新晓 曾秀文 张涛 黄俏婷 |
| 作者单位： | 1. 惠州学院生命科学系,广东,惠州,516007 2. 惠州市农业科学研究所,广东,惠州,516007 3. 惠州市惠城区蔬菜研究所,广东,惠州,516007 |
| 基金项目： | 广东省省级产业技术研究与开发项目[粤财工(2008)151号]、惠州市科技计划项目 |
| 摘 要： | 利用ISSR技术对3个甜玉米组合及其父、母本自交系共9个材料进行鉴定，从30条引物中筛选出4条扩增条带清晰、多态性较高的引物，共扩增出31条DNA带，其中24条DNA带具有多态性，多态性比率为77.4%。对9个玉米材料的亲缘关系进行聚类分析，9个甜玉米材料分为两个类群。 |
| 关 键 词： | 甜玉米；ISSR；纯度鉴定 |
| 收稿时间： | 2008-07-29 |
| 修稿时间： | 2008-12-12 |
Studies Purity Identification of Sweet Corn Seeds UsingISSR Molecular Marker Technique |
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| CHEN Zhao-gui,JIANG Xin-xiao,ZENG Xiu-wen,ZHANG Tao,HUANG Qiao-ting. Studies Purity Identification of Sweet Corn Seeds UsingISSR Molecular Marker Technique[J]. Journal of Maize Sciences, 2010, 18(1): 46-48 | |
| Authors: | CHEN Zhao-gui JIANG Xin-xiao ZENG Xiu-wen ZHANG Tao HUANG Qiao-ting |
| Abstract: | To study purity identification of sweet corn seeds, ISSR markers were used to screen in 9 materials of sweet corn. Four primers were screened out from 30 ISSR primers totally to amplify the sweet corn genome DNAs of 9 materials effectively and 31 sites were amplified out totally, including 24 polymorphism sites with polymorphism rate of 77.4%. All of the 9 materials could be differentiated from each other based on the fingerprints established by these specific bands. The systematical cluster analysis conducted with UPGMA showed that 9 sweet corn materials could be divided into two groups. |
| Keywords: | ISSR Sweet corn ISSR Purity identification |
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