抗真菌单体组分DZP8对水稻纹枯病菌的抑菌机制

为探明链霉菌702菌株所产抗真菌单体组分DZP8对水稻纹枯病菌的抑菌机制,在显微镜下观察DZP8对水稻纹枯病菌菌丝形态的影响,并测定DZP8对水稻纹枯病菌内含物渗漏的影响以及对细胞膜的损伤。结果表明,在液体培养条件下,抗真菌单体组分DZP8能够引起水稻纹枯病菌发生一系列菌丝形态变化,1.81μg/ml和3.35μg/ml DZP8处理24 h可使水稻纹枯病菌菌丝内出现大液泡,菌丝表面粗糙,内含物增多,随着作用时间的延长,菌丝逐渐扭曲并呈现出不规则缢缩。20.10μg/ml DZP8处理可导致菌丝培养液电导率增加,可溶性糖和蛋白质渗漏,菌丝膜脂质过氧化。DZP8在较低浓度(1.81μg/ml)下就能够引起水稻纹枯病菌菌丝形态的变化,但只有在较高浓度(20.10μg/ml)时才造成细胞膜的显著损伤,说明细胞膜可能为DZP8的一个作用位点,同时还可能存在其他的作用方式。

Inhibition mechanism of antifungal monomers DZP8 against Rhizoctonia solani

In order to study the inhibition mechanism of antifungal monomers DZP8 produced by streptomyces 702 strain against Rhizoctonia solani,the effects of DZP8 on mycelial morphology were investigated by light microscope,and the effects of DZP8 on mycelial inclusions leakage and cell membrane damage of mycelium were determined.The results showed that DZP8 caused a series of changes in hyphae morphology of Rhizoctonia solani in liquid culture condition.1.81 μg/ml or 3.35 μg/ml DZP8 treatment for 24 h caused big vacuole,rough surface and more inclusions of mycelium.With the treatment time prolonging,the mycelium distorted and appeared irregular constriction.20.10 μg/ml DZP8 treatment led to the increase of electrical conductivity of mycelium culture medium,leakage of soluble sugar and protein,and the lipid peroxidation of mycelium membrane.It was found that DZP8 at a very low concentration could cause Rhizoctonia solani hyphal morphological change,while only a certain concentration could cause significant damage to cell membrane,which indicated that cell membrane was possible one of the action sites of DZP8,and it might have other action sites or mechanism.