| 高纤维强力棉花种质系苏远7235 BAC文库的构建 |
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| 引用本文： | 王省芬,马骏,马峙英,张桂寅,郑拥民.高纤维强力棉花种质系苏远7235 BAC文库的构建[J].棉花学报,2006,18(4):200-203. |
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| 作者姓名： | 王省芬 马骏 马峙英 张桂寅 郑拥民 |
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| 作者单位： | 河北农业大学/河北省作物种质资源重点实验室,河北,保定,071001 |
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| 基金项目： | 国家研究发展基金;国家高技术研究发展计划(863计划);教育部科学技术研究项目 |
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| 摘 要： | 苏远7235是我国利用异常棉等多个野生种创造的高纤维强力棉花种质系,是开展棉花纤维品质研究的重要材料。本研究以pIndigoBAC-5(HindIII-cloning ready)为载体,构建了苏远7235的细菌人工染色体(Bacterial Artificial Chromosome,BAC)文库,该文库包含30336个BAC克隆。分析结果表明,重组克隆苏远7235 DNA插入片段为50-140 kb,平均120 kb,空载率2.1%,89.6%的克隆插入片段大于100 kb。
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| 关 键 词： | 棉花 纤维品质 细菌人工染色体文库 |
| 文章编号： | 1002-7807(2006)04-0200-04 |
| 修稿时间： | 2005年12月6日 |
BAC Library Construction and Characterization of Suyuan 7235, A Cotton Germplasm with High Fiber Strength |
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| WANG Xing-fen,MA Jun,MA Zhi-ying,ZHANG Gui-yin,ZHENG Yong-min.BAC Library Construction and Characterization of Suyuan 7235, A Cotton Germplasm with High Fiber Strength[J].Cotton Science,2006,18(4):200-203. |
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| Authors: | WANG Xing-fen MA Jun MA Zhi-ying ZHANG Gui-yin ZHENG Yong-min |
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| Abstract: | In this study,bacterial artificial chromosome(BAC) library of Suyuan7235,a cotton germplasm with high fiber strength has been constructed following the partial digestion of genomic DNA with HindIII.The pIndigoBAC-5(HindIII-cloning ready) cloning vector was used for the library.The BAC library included 30336 clones.Analysis of 96 recombinants showed that the insert DNA size ranged from 50 to 140 kb,averaged 120kb with less than 2.1% of empty clones.As much as 89.6% clones had inserts over 100 kb.As genomic resources,the library has potential use in further research of fiber strength gene analysis and gene cloning,as well as SSR primer development. |
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| Keywords: | cotton fiber quality bacterial artificial chromosome(BAC) library |
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