| 颐和园古桑树的组织培养与快速繁殖 |
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| 引用本文： | 郑广顺 王瑾瑜 戴全胜 车晓航 李睿 陈玉珍 卢存福. 颐和园古桑树的组织培养与快速繁殖[J]. 中国农学通报, 2011, 27(8): 32-35 |
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| 作者姓名： | 郑广顺 王瑾瑜 戴全胜 车晓航 李睿 陈玉珍 卢存福 |
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| 作者单位： | 1. 林木育种国家工程实验室/教育部林木花卉遗传育种重点实验室/北京林业大学生物科学与技术学院,北京,100083
2. 北京市颐和园管理处,北京,100091 |
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| 基金项目： | 北京市教委共建项目; 北京市公园管理处课题资助 |
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| 摘 要： | 以北京颐和园古桑树为外植体进行组织培养，取桑树当年新生嫩茎切段为外植体进行，其中不定芽启动培养基为MS+6-BA 0.5 mg/L+NAA 0.1 mg/L+PVP 0.2 g/L；将无菌苗接到最适分化培养基MS+ 6-BA 0.8 mg/L +NAA 0.1 mg/L +PVP 0.2 g/L上，组培苗分化率高；不定根最适诱导培养基为：1/2MS+NAA 0.5 mg/L +PVP 0.2 g/L，生根率达100%。组培苗移栽成活率达95%。
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| 关 键 词： | 黄鳝 黄鳝 |
| 收稿时间： | 2010-10-11 |
| 修稿时间： | 2010-12-30 |
In vitro Propagation of Ancient Mulberry(Morus alba L.)from the Summer Palace in Beijing |
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| Zheng Guangshun,Wang Jinyu,Dai Quansheng,Che Xiaohang,Li Rui,Chen Yuzhen,Lu Cunfu. In vitro Propagation of Ancient Mulberry(Morus alba L.)from the Summer Palace in Beijing[J]. Chinese Agricultural Science Bulletin, 2011, 27(8): 32-35 |
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| Authors: | Zheng Guangshun Wang Jinyu Dai Quansheng Che Xiaohang Li Rui Chen Yuzhen Lu Cunfu |
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| Affiliation: | Zheng Guangshun1,Wang Jinyu1,Dai Quansheng2,Che Xiaohang2,Li Rui1,Chen Yuzhen1,Lu Cunfu1 (1National Engineering Laboratory for Tree Breeding/Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants Ministry of Education/College of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing 100083,2Administrative Office of the Summer Palace,Beijing 100091) |
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| Abstract: | Nodal explants derived from ancient mulberry (Morus alba ),which was lived in the Summer Palace for more than 100 years,were used to initiate shoot cultures.Bud formation on the nodal explants was induced on an optimal medium MS+6-BA 0.5 mg/L+NAA 0.1 mg/L+PVP 0.2 g/L.To get a high propagation ratio,the seedlings were transferred to the medium of MS+ 6-BA 0.8 mg/L +NAA 0.1 mg/L +PVP 0.2 g/L.The optimal medium for adventitious root inducing was 1/2MS+NAA 0.5 mg/L +PVP 0.2 g/L,and the rooting rate was 100%.The... |
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| Keywords: | Summer Palace Morus alba nodal explants tissue culture |
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