The use of MPB70 and MPB83 to distinguish between bovine tuberculosis and paratuberculosis |
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| Authors: | Carla D Marassi Jim McNair John Pollock Paula Ristow Leila S Fonseca Walter MR Oelemann Walter Lilenbaum |
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| Institution: | a Laboratory of Veterinary Bacteriology, Universidade Federal Fluminense, Brazil;b Agri-Food and BioSciences Institute, Veterinary Sciences Division, Stormont, Ireland;c Microbiology Institute, Universidade Federal do Rio de Janeiro, Brazil |
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| Abstract: | In order to demonstrate the potential to distinguish paratuberculosis (PTB) from bovine tuberculosis infection (TB), ELISAs with M. bovis-specific MPB70 or MPB83 as capture antigens were developed and tested on two groups of cattle: Group A comprised 23 animals positive for Mycobacterium avium paratuberculosis (Map) and TB free. Group B comprised 48 animals from a Map free herd during the previous 5 years, but confirmed as tuberculous by positive results on PPD testing and M. bovis culture. Results demonstrated a significant difference (p < 0.01) between reactivity of sera from these groups, encouraging the study of purified proteins to differentiate between both diseases. |
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| Keywords: | Johne's disease Paratuberculosis Bovine tuberculosis MPB70 MPB83 ELISA |
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