| 一株产赖氨酸芽孢杆菌的筛选 |
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| 引用本文： | 邢月腾,李丽立,王升平,范觉鑫,林彬彬,欧荣娣,张彬. 一株产赖氨酸芽孢杆菌的筛选[J]. 养猪, 2014, 0(3): 9-10 |
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| 作者姓名： | 邢月腾 李丽立 王升平 范觉鑫 林彬彬 欧荣娣 张彬 |
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| 作者单位： | [1]湖南农业大学动物科学技术学院,湖南长沙410128 [2]中国科学院亚热带农业生态研究所动物生态营养与健康养殖联合实验室,湖南长沙410125 |
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| 基金项目： | 国家自然科学基金项目（31272462）;湖南省科技计划重点项目（2100CK2006）;长沙市科技计划重点项目（K1201108-21） |
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| 摘 要： | 87株芽孢杆菌经过2次菌液上清液赖氨酸含量测定，筛选出1株赖氨酸产量较高的菌株YB83，其在LB上清发酵液中赖氨酸含量达186.85μg/mL，比对照组提高27.68%，该菌通过菌落形态、生理生化特征和分子生物学鉴定为枯草芽孢杆菌。
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| 关 键 词： | 芽孢杆菌 赖氨酸 筛选 |
Screening of Bacillus spp. Producing Lysine |
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| XING Yueteng,LI Lili,WANG Shengping,FAN Juexin,LIN Binbin,OU Rongdi,ZHANG Bin. Screening of Bacillus spp. Producing Lysine[J]. Swine Production, 2014, 0(3): 9-10 |
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| Authors: | XING Yueteng LI Lili WANG Shengping FAN Juexin LIN Binbin OU Rongdi ZHANG Bin |
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| Affiliation: | 1.College of Animal Science and Technology,Hunan Agricultural University,Changsha,410128,China; 2.Laboratory of Animal Nutrition and Human Health Laboratory,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha,410125,China) |
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| Abstract: | 87 strains of Bacillus spp. as initial bacteria were applied to select and breed high-yield lysine strain. After twice screening by determining the lysine content of bacterial supermatant,one strain named YB83 was screened out,whose lysine content was 186.85 μg/mL,27.68% higher than LB control medium.According to the morphological and physiochemical properties,combining with 16S rDNA gene sequence and its position in the phylogenetic tree,strain YB83 was finally identified as Bacillus subtilis. |
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| Keywords: | Bacillus spp. Lys screening |
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