1株广东鸡源新城疫病毒的分离鉴定和毒力基因分析 |
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引用本文: | 黎先伟,;钟植文,;陈珊,;吴晓禅,;刘镇明,;杨傲冰. 1株广东鸡源新城疫病毒的分离鉴定和毒力基因分析[J]. 畜牧兽医科技信息, 2014, 0(4): 34-37 |
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作者姓名: | 黎先伟, 钟植文, 陈珊, 吴晓禅, 刘镇明, 杨傲冰 |
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作者单位: | [1]广东永顺生物制药股份有限公司,广东广州511356; [2]华南农业大学兽医学院,广东广州510642 |
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摘 要: | 从广东粤东地区某商品蛋鸡场的发病鸡群中分离到1株病毒(YS株),经血凝和血凝抑制试验确定为新城疫病毒(NDV).对该分离株的F蛋白氨基酸序列的分析结果表明,其F0裂解位点的氨基酸序列为1nR-R-Q-K-R-F117,且含有101K和121V,符合典型NDV强毒株的分子特点.遗传分析结果显示分离株属于基因Ⅶd亚型.F和HN基因的氨基酸相似性分析表明,分离株与基因Ⅶ型NDV Chicken/China/Shandong/02/2010株的相似性最高,均达到99%,而与常用疫苗株B1、V4、Clone30、Mukteswar和La Sota的相似性则较低,分别在86.8%~ 90.4%和87.2%~88.3%之间,说明分离株与经典的NDV毒株存在一定的差异.
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关 键 词: | 分离 基因Ⅶ型 鸡 |
Identification of One Newcastle Disease Viruse Strain and its Virulent Genes |
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Affiliation: | Li Xianwei, Zhong Zhiwen, Chen Shan, Wu Xiaochan, Liu Zhenming, Yang Aobing ( 1.Guangdong Win-sun Bio-pharmaceutical Co., Ltd, Guangzhou 511356, China; 2.College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China ) |
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Abstract: | In recent years, atypical Newcastle disease was popular in some domestic areas. Samples from some sick commercial layer from eastern Guangdong, were inoculated to 9-11 day-old chicken embryos, and one virus, named YS, was isolated. HA and HI tests showed Newcastle disease virus positive. The result of sequence analysis showed that the amino-acid sequence of the fusion protein cleavage site was 112R-R-Q-K-R-F117, with 101 K and 121 V, a typical sequence ofvelogenic strains. Genetic analysis showed that the isolate belonged to genotype VII. The homology analysis of F and HN gene showed that the isolate strain had highest identities with the genotype VII NDV (Chicken/China/Shandong/02/2010), both with 99%, but low identities with the common vaccine strains, such as B 1, V4, Clone30, Mukteswar and LaSota, only with 86.8%- 90.4% and 87.2%-88.3%, respectively, which differed from the classical strains of NDV. |
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Keywords: | Newcastle disease virus F gene HN gene genotype VII velogenic strain |
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