基于二代测序的集群分离分析法在木本植物基因定位中的应用

植物重要性状相关的数量性状基因座（QTL）和基因定位一直是结构基因组学的研究重点，也是遗传机制解析和分子育种的重要基础。木本植物具有重要的经济、生态价值，但用于其研究的传统基因定位方法存在耗时长、工作量大、通量低、成本高且效果不佳等问题。近年来，由于测序技术发展日新月异，基于二代测序技术的集群分离分析法（NGS-based BSA）开始应用于木本植物中。与传统的集群分离分析法（BSA）技术相比，NGS-based BSA周期短、效率高，能对质量或数量性状进行精确定位，在木本植物遗传学和组学研究方面具有广阔的应用前景。文中简述了BSA的发展历程和研究策略，综述了其在木本植物研究中的进展，分析了NGS-based BSA的优势及存在的问题，展望了其在木本植物遗传学、组学和种质改良领域中的应用潜力。

Application of Next-generation Sequencing Based Bulked Segregant Analysis in Gene Mapping of Woody Plants

Mapping of functional QTLs and genes relevant to plant traits is always the focus of structural genomics, which also lays a solid foundation for the genetic mechanism deciphering and molecular breeding. Woody plants have significant economic and ecological values. However, the common genetic mapping approaches adopted for woody plant analysis are time-consuming, laboring, low-fluxes, costly and inefficient. Recently, next-generation sequencing-based bulked segregant analysis (BSA) has been used in woody plant QTL and gene mapping with the rapid development of the sequencing technology. Compared with conventional BSA, it has the advantages like short time period, high efficiency and accurate positioning of quality or quantitative traits, which has great potential to be used in the genetics and genomics of woody plants. The paper summarizes the development and strategies of BSA, reviews its latest research progresses in woody plants, analyzes its advantages and extant problems, and prospects its potential to be applied in genetics, genomics and germplasm improvement in woody plants.