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林木体细胞胚胎发生机理研究进展
引用本文:吴高殷,韦小丽,王晓,刘盈盈,陈胜群.林木体细胞胚胎发生机理研究进展[J].世界林业研究,2019,32(5):9-14.
作者姓名:吴高殷  韦小丽  王晓  刘盈盈  陈胜群
作者单位:贵州大学林学院, 贵阳 550025
基金项目:国家自然科学基金“花榈木根瘤菌多样性及幼苗形成调控因素研究”(31460193);贵州省高层次创新型人才培养计划“百”层次人才项目“贵州珍贵树种栽培生理生态及精准化育苗技术研究”(黔科合平台人才[2016]5661);贵州省林业厅项目“贵州优质乡土阔叶树种培育与示范”[黔林科合(2010)重大02]。
摘    要:体细胞胚胎发生技术能够实现优良林木品种的规模化繁育及优良性状保留,为林木扩繁、种质资源保存、遗传改良和机理研究等提供材料。但其仍存在诱导率低、褐化现象严重、愈伤分化困难和再生植株生长较弱等问题。文中从生理生化学、细胞生物学和分子生物学3个方面阐述体胚发生机理,包括内源激素含量、细胞程序性死亡和体胚发生相关基因等,并在当前研究基础上对未来林木体胚发生深入研究进行展望,旨在为建立高效林木体胚发生体系及其机理研究提供参考。

关 键 词:体细胞胚胎发生    生理生化学    细胞生物学    分子生物学
收稿时间:2018/11/13 0:00:00
修稿时间:2019/5/26 0:00:00

Research Progress on Mechanism of Somatic Embryogenesis in Forest Trees
Wu Gaoyin,Wei Xiaoli,Wang Xiao,Liu Yingying and Chen Shengqun.Research Progress on Mechanism of Somatic Embryogenesis in Forest Trees[J].World Forestry Research,2019,32(5):9-14.
Authors:Wu Gaoyin  Wei Xiaoli  Wang Xiao  Liu Yingying and Chen Shengqun
Institution:College of Forestry, Guizhou University, Guiyang 550025, China
Abstract:Somatic embryogenesis (SE) is an effective way for large-scale breeding and good traits retention of superior tree species, which provides materials for tree propagation, germplasm conservation, genetic improvement and mechanism research. However, SE still has problems such as low induction rate, severe browning, difficulty of callus differentiation and weak growth of regenerated plants. This paper explains the mechanism of SE from physiological biochemistry, cell biology and molecular biology, including endogenous hormone content, programmed cell death (PCD) and key genes of somatic embryogenesis, and put forward the future development direction of forest tree SE based on current researches, which is aimed at providing references for establishing an efficient system of forest tree SE and related mechanism research.
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