小麦对白粉菌过敏性反应与H_2O_2及3种氧化酶活性变化的关系(英文)

[目的]研究小麦对白粉菌过敏性反应与H2O2及3种氧化酶活性变化的关系,为探讨小麦对白粉菌抗性生理机制奠定基础。[方法]以白粉菌菌株17(Bgt17)和菌株6(Bgt6)及小麦品种扬158为试验材料,测定H2O2处理的小麦叶片过敏性细胞数量及POD、PPO、SOD酶活性。POD活性测定参照Hammershmidt方法并加以改进,用0.05mmol/LpH值为5.5的磷酸缓冲液进行提取,以愈创木酚作为底物测定,以1minA470变化0.01为1个酶活力单位;PPO活性测定参照ConstableandRyan方法进行,用0.05mmol/LpH值为5.5的磷酸缓冲液提取,以儿茶酚为底物测定,以1minA525变化0.01为PPO活力单位;SOD活性的测定参照Levine等方法进行,用提取液(50mmol/LMet,0.1mol/LpH值为7.8的磷酸缓冲液,1mmol/L二硫苏糖醇(DDT))提取。测定SOD对氮蓝四唑(NBT)光还原的抑制率,以抑制NBT光还原50%为1个酶活力单位。[结果]乳突在亲和性与非亲和性组合叶表细胞形成的差异不大,非亲和性反应叶表细胞过敏性反应发生早且明显比亲和性反应多;接种Bgt17后不同时间及非亲和性组合中含H2O2的过敏性反应细胞频率明显高于亲和性组合;用5、10、20mmol/L抗坏血酸处理后,小麦叶片中过敏性细胞数量显著减少,15.0与30.0mmol/LH2O2处理后诱导亲和性反应小麦叶片中过敏性细胞数量显著增加;接种白粉菌后,小麦叶片中POD、PPO、SOD活性均呈先上升后下降趋势,且非亲和性组合比亲和性组合3种酶的活性高。[结论]HO、PPO、SOD活性与小麦对白粉菌抗性存在一定相关性。

Relationship between the Hypersensitive Response of Wheat to Blumeria graminis f. sp. tritici and Hydrogen Peroxide, Three Enzyme Activities Changes

[Objective] The research aimed to study the relationship between the hypersensitive response of wheat to Blumeria graminis f.sp.tritici and hydrogen peroxide,3 enzyme activities changes and lay the foundation for discussing the resistant physiological mechanism of wheat to B.graminis.[Method] Taking B.graminis Bgt 17 and Bgt 6 and wheat cultivar Yang 158 as test materials,the number of hypersensitive cells and activities of POD,PPO and SOD in wheat leaves treated by H2O2 were determined.[Result] The mastoid...