家蚕病原性微孢子虫的蛋白质化学性质的研究

抽提了微 孢子虫的总 蛋白和选择 性分离纯 化了主要 蛋白组 分。对 总蛋白 进行了 酸性 Ｐ Ａ Ｇ Ｅ、碱性 Ｐ Ａ Ｇ Ｅ、 Ｓ Ｄ Ｓ Ｐ Ａ Ｇ Ｅ 和氨基酸组成分析,并对 Ｓ Ｄ Ｓ Ｐ Ａ Ｇ Ｅ 结果进行了薄层扫描分析。发现每一样品总蛋白在 Ｓ Ｄ Ｓ Ｐ Ａ Ｇ Ｅ 图谱上均分离出３０ 多条蛋白带,均有５ 条主带,但位置不同,各条蛋白带的相对含量不同。氨基酸组成分析结果中发现各种孢子总蛋白所含的氨基酸种类基本相同,均含有１６ 种氨基酸,但各种氨基酸的相对含量不同。对主要蛋白组分进行了 Ｓ Ｄ Ｓ Ｐ Ａ Ｇ Ｅ 氨银染色法纯度鉴定、薄层扫描分析和氨基酸组成分析,发现彼此之间有共同点,但也存在一定的差异。

Studies on Sporal Protein Chemical Properties of Microsporidias Pathogenic to Silkworm,Bombyx mori

Total proteins of microsporidias were extracted and its major components were selectively separated and purified.the total proteins were analysed with acid PAGE,alkaline PAGE and SDS PAGE.Moreover,the SDS PAGE gels were scanned by Imaging Densitometer.The protein profiles of each microsporidia are complex.There are more than 30 bands and 5 major bands on the SDS PAGE profiles of each microsporidia.But the places of major bands and the relative contents of each protein band are different.The compositions of amino acids of the sporal proteins are analysed.They are similar among these microsporidias.There are 16 kinds of amino acids in the total proteins of each microsporidia,but their relative contents are different.The major components were identified by silver dyed SDS PAGE and the SDS PAGE gels were scanned.The composition of all amino acids was also analysed.There are not only common ground,but also certain differences among them.