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| 体外盐酸处理与PIN对酯酶A4活性影响的关系 | |
| 引用本文： | 徐世清,司马杨虎,郑必平,虞晓华,甲斐英则,徐俊良. 体外盐酸处理与PIN对酯酶A4活性影响的关系[J]. 蚕业科学, 1999, 25(3): 149-153 |
| 作者姓名： | 徐世清 司马杨虎 郑必平 虞晓华 甲斐英则 徐俊良 |
| 作者单位： | 1. 苏州大学蚕桑学院,苏州,215151 2. 日本国立鸟取大学农学部 3. 浙江大学蚕学系 |
| 摘 要： | 从家蚕Ｃ１０８ 品种产下后２ｄ 的滞育性卵纯化酯酶Ａ４ 及其活性抑制多肽ＰＩＮ,体外调查了盐酸处理与ＰＩＮ 对酯酶Ａ４ 的ＡＴＰａｓｅ 活性影响的关系。结果表明：盐酸处理不仅迅速消除了ＰＩＮ 对酯酶Ａ４ 的ＡＴＰａｓｅ 活性抑制作用;盐酸处理使蚕卵酯酶Ａ４ 的ＡＴＰａｓｅ 活性峰提早出现的时间还反映了蚕卵的滞育发育时间。 |
| 关 键 词： | 家蚕 滞育性卵 盐酸处理 酯酶A4 多肽抑制栓 |
| 修稿时间： | 1998－05－15 |
Changes of Esterase A4 Activity and Its Relationship With Acid-Treatment After the Enzymic Protein Treated by PIN |
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| Xu Shiqing,Sima Yanghu,Zheng Biping,Yu Xiaohua,Kai Hidenori,Xu Junliang. Changes of Esterase A4 Activity and Its Relationship With Acid-Treatment After the Enzymic Protein Treated by PIN[J]. Acta Sericologica Sinica, 1999, 25(3): 149-153 | |
| Authors: | Xu Shiqing Sima Yanghu Zheng Biping Yu Xiaohua Kai Hidenori Xu Junliang |
| Abstract: | Esterase A4(Ease A4) and the peptidic inhibitory needle(PIN) was purified from silkworm eggs of C108 2 days after oviposition,and the effects of hydrochloric acid treatment and PIN on ATPase activity of Ease A4 were investigated in vitro .The results showed that hydrochloric acid treatment eliminated the inhibition of PIN on ATPase activity of Ease A4 quickly;Moreover,the time of ATPase activity peak appearance moved up by hydrochloric acid treatment interrelated to the diapause development of silkworm eggs. |
| Keywords: | Bombyx mori Diapause eggs Hydrochloric acid treatment Eaterase A4(Ease A4) Peptidic inhibitory needle(PIN) |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |