| UPLC法测定西洋参中7种人参皂苷含量 |
| |
| 引用本文： | 逄世峰,孙成贺,李亚丽,赵景辉,王英平,张志东,李学军,李显辉.UPLC法测定西洋参中7种人参皂苷含量[J].人参研究,2013(4):9-11. |
| |
| 作者姓名： | 逄世峰 孙成贺 李亚丽 赵景辉 王英平 张志东 李学军 李显辉 |
| |
| 作者单位： | [1]中国农业科学院特产研究所,吉林长春130112 [2]康美新开河（吉林）药业有限责任公司,吉林集安134200 |
| |
| 基金项目： | 国家科技支撑计划项目,项目编号：2011BA103B0106. |
| |
| 摘 要： | 建立同时测定西洋参中7种人参皂苷含量的快速检测方法.方法：采用超高效液相色谱法,ACQUITY UPLC BEH C18色谱柱（2.1 ×50mm,1.7μm）,以乙腈-水为流动相,梯度洗脱,流速：0.5ml/min,柱温35℃,进样体积2μL,检测波长203 nm.结果：人参皂苷Rg1、Re、Rb1、Rc、Rb2、Rb3、Rd存在良好线性关系,线性范围分别为0.0067～0.334mg/ml,0.0151～0.756 mg/ml,0.0130～0.649 mg/ml,0.0043～0.217 mg/ml,0.0044～0.221mg/ml,0.0085～0.424 mg/ml,0.0086～0.43 mg/ml.结论：该方法准确、重现性好,可用于西洋参中人参皂苷含量测定.
|
| 关 键 词： | 超高效液相色谱（UPLC） 西洋参 人参皂苷 |
Determination of Seven Ginsenosides in Root of Panax quinguefolium L.by UPLC |
| |
| Institution: | PANG Shi feng SUN Cheng he LI Ya li ZHAO Jing hui WANG Ying ping ZHANG ZHi dong LI Xue jun(Institute of Special Wild Economic Animal and Plant Science, CA A S ,Jilin , Changchun , 130112)(Kang Mei the Xinkai River (Jilin) Pharmaceutical Co., Ltd., Jilin Ji＇an 134200) |
| |
| Abstract: | To develop the method for the content determination of seven ginsenoside in Panax ginseng. Method：This assay was performed on ACQUITY BEH C18（2.1 mm ×50 mm, 1.7 μm） column with acetonitr/le-water as mobile phase in gradient elution at a flow rate of 0.5mL min-1.The column temperature was 35℃.The injection volume was 2μL.And the detection wavelength was 203 nm. Result： There was a good linear relationship among ginsenosides Rg 1, Re, Rb 1, Rc, Rb2, Rb3, Rd in 0.0067 -0.334mg/ml,0.0151-0.756 mg/ml,0.0130 -0.649 mg/ml,0.0043 -0.217 mg/ml,0.0044- 0.221mg/ml,0.0085-0.424 mg/ml,0.0086-0.43 mg/ml respectively. Conclusion：this method is accurate and good reproducibility suitable for the measurement of ginsenosides in the roots of Panax quinguefolium L. |
| |
| Keywords: | Uhra performance liquid chromatography Panax quinguefolium L Ginsenoside |
| 本文献已被 维普 等数据库收录！ |
|
