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植物内生细菌yc8对油菜菌核病菌抑菌作用的初步研究
引用本文:任璐,刘慧平,韩巨才,邢鲲.植物内生细菌yc8对油菜菌核病菌抑菌作用的初步研究[J].安徽农业科学,2007,35(11):3294-3295.
作者姓名:任璐  刘慧平  韩巨才  邢鲲
作者单位:山西农业大学农学院,山西太谷,030801
基金项目:山西省自然科学基金 , 山西省留学回国人员科研启动基金 , 山西省科技厅资助项目
摘    要:测定了植物内生细菌yc8对油菜菌核病菌的抑菌活性.研究结果表明,yc8对油菜菌核病菌菌丝有强烈的溶菌作用,EC50为10.14%.光学显微镜观察证实,yc8发酵液处理后可造成病原菌细胞畸形、胞内物质外泄和细胞壁崩溃.植物离体组织抑菌作用测定结果表明.先喷发酵液后接菌比先接菌后喷发酵液防治效果更明显.

关 键 词:植物内生细菌  油菜菌核病菌  抑菌作用
文章编号:0517-6611(2007)11-03294-02
收稿时间:2007-01-19
修稿时间:2007-01-19

Preliminary Study on Antagonistic Action of Endophyte Bacteria yc8 to Sclertinia sclerotiorum
REN Lu ,et al.Preliminary Study on Antagonistic Action of Endophyte Bacteria yc8 to Sclertinia sclerotiorum[J].Journal of Anhui Agricultural Sciences,2007,35(11):3294-3295.
Authors:REN Lu  
Institution:School of Agronomy, Shanxi Agricultural University, Taigu Shanxi 030801
Abstract:The inhibitory activity of entophytic bacteria yc8 isolated from rape to Sclertinia sclerotiorum was surveyed.The results showed that the fermentation liquid of yc8 could strongly inhibit the hypha growth of pathogen.And EC50 value was 10.14 %.Observation under microscope found out that the substances excreted by endophyte bacteria yc8 could cause S.a sclerotiorum cells deformities,cell wall collapse and the material inside cell wall leaking.The yc8 had strong effect on the hypha of S.sclerotiorum.Results of plant tissue tests in vitro showed that spraying with fermentation liquid before putting on the pathogen could effectively control the disease.
Keywords:Endophytic bacteria  Sclertinia sclerotiorum  Antagonistic function
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