向日葵S-腺苷甲硫氨酸合成酶基因克隆与分析

为了研究S-腺苷甲硫氨酸合成酶(S-adenosylmethionine synthetase,SAMS)在向日葵(He-lianthus annuus)抗旱和耐盐过程中的作用,先根据计算机辅助克隆结果设计引物,抽提盐胁迫向日葵叶片的总RNA,然后采用RT-PCR扩增技术克隆了向日葵的1个SAMS基因(命名为HaSAMS1),HaSAMS1基因的编码序列长1173bp,编码390个氨基酸残基。HaSAMS1没有跨膜结构域,没有信号肽,含有SAMS蛋白的特征序列。HaSAMS1与其他物种的SAMS具有较高的序列相似性,与茼蒿(Chrysanthemum coronarium)和拟南芥(Arabidopsis thaliana)等高等植物SAMS的氨基酸序列一致性介于86.8%~96.9%。HaSAMS1基因的克隆为进一步研究向日葵抗旱和耐盐机理奠定了基础。

Molecular Cloning and Bioinformatic Analysis of S-adenosylmethionine Synthetase Gene from Helianthus annuus

To investigate contributions of S-adenosylmethionine synthetase(SAMS) to tolerance to drought and salt in Helianthus annuus,we cloned one SAMS gene(designated as HaSAMS1) using RT-PCR from H.annuus.The primers were designed according to results of computer-assisted cloning.Total RNAs were isolated from the leaves of H.annuus treated by salt.The HaSAMS1 gene contained a complete coding sequence of 1173 bp,encoding the protein of 390 amino acids.HaSAMS1 contained signature motifs of SAMS family,but had no mem...