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| 大豆品种合丰25体细胞胚发生条件的优化 | |
| 引用本文： | 李宁辉,徐鹏飞,范素杰,吴俊江,李文滨,王萍,王欣,姜良宇,王金生,张淑珍. 大豆品种合丰25体细胞胚发生条件的优化[J]. 作物杂志, 2011, 0(4): 23-28 |
| 作者姓名： | 李宁辉 徐鹏飞 范素杰 吴俊江 李文滨 王萍 王欣 姜良宇 王金生 张淑珍 |
| 作者单位： | 1. 东北农业大学大豆研究所;大豆生物学教育部重点实验室,150030,黑龙江哈尔滨 2. 黑龙江省农业科学院 大豆研究所,150086,黑龙江哈尔滨 3. 淮海工学院海洋学院,222005,江苏连云港 |
| 基金项目： | 教育部新世纪人才培养计划，国家自然科学基金，黑龙江省自然科学基金，黑龙江省新世纪优秀人才培养计划，中国博士后基金，黑龙江省博士后基金，博士后落户黑龙江科研启动基金资助项目 |
| 摘 要： | 为优化大豆体细胞胚再生植株的条件,提高大豆遗传转化的效率,本研究选用大豆品种合丰25为试验材料,针对影响体细胞胚诱导的3个关键因素pH值、2,4-D浓度及光照条件进行体细胞胚发生频率的研究。结果表明:pH值、2,4-D浓度、光照条件这3种因素的不同处理对体细胞胚的诱导影响显著,其幼胚的发生频率最高为70.70%、最低为3.70%。当pH值为7.0、2,4-D浓度为20mg/L,黑暗培养最有利于合丰25体细胞胚的诱导,其胚的发生频率为70.70%,明显高于其他处理。 |
| 关 键 词： | 合丰25 体细胞胚 大豆再生 优化 |
Optimizing of Regeneration Conditions of Somatic Embryo for Soybean Variety "Hefeng 25" |
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| Li Ninghui,Xu Pengfei,Fan Sujie,Wu Junjiang,Li Wenbin,Wang Ping,Wang Xin,Jiang Liangyu,Wang Jinsheng,Zhang Shuzhen. Optimizing of Regeneration Conditions of Somatic Embryo for Soybean Variety "Hefeng 25"[J]. Crops, 2011, 0(4): 23-28 | |
| Authors: | Li Ninghui Xu Pengfei Fan Sujie Wu Junjiang Li Wenbin Wang Ping Wang Xin Jiang Liangyu Wang Jinsheng Zhang Shuzhen |
| Affiliation: | Li Ninghui1,Xu Pengfei1,Fan Sujie1,Wu Junjiang2,Li Wenbin1,Wang Ping3,Wang Xin1,Jiang Liangyu1,Wang Jinsheng2,Zhang Shuzhen1(1Soybean Research Institute of Northeast Agricultural University,Key Laboratory of Soybean Biology in Chinese Ministry of Education,Harbin 150030,Heilongjiang,2Soybean Research Institute of Heilongjiang Academy of Agriculture Sciences,3School of Marine Science and Technology,Huaihai Institute of Technology,Lianyungang 222005,Jiangsu,China) |
| Abstract: | To optimize regeneration conditions of soybean somatic embryo and to improve efficiency of soybean genetic transformation,the pH value,2,4-D concentration and illumination condition were studied using Hefeng 25 as the test material.The results showed that the different treatments of the three factors had a significant influence on the development of somatic embryo.The highest efficiency was 70.70%,while the lowest efficiency was 3.70%.When the pH value was 7.0,2,4-D concentration was 20mg/L and the material... |
| Keywords: | Hefeng 25 Somatic embryos of soybean Soybean regeneration Optimization |
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