| 冷季型草坪草基因组DNA的提取方法比较 |
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| 引用本文： | 邱永福,田志宏,杨晓松.冷季型草坪草基因组DNA的提取方法比较[J].长江大学学报,2005,2(2):28-30,38. |
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| 作者姓名： | 邱永福 田志宏 杨晓松 |
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| 作者单位： | 长江大学生命科学学院,湖北荆州434025 |
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| 基金项目： | 湖北省科技发展计划
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湖北省教育厅重大科技攻关项目 |
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| 摘 要： | 采用SDS法、热CTAB法、高盐低pH法和改进CTAB法分别提取高羊茅(Festuca arundinoceaSchreb.)、草地早熟禾(Poa pratensis L.)和多年生黑麦草(Lolium perenne L.)3种冷季型草坪草基因组DNA.结果表明,改进CTAB法为最佳的提取方法,分离的DNA产率高、纯度高、质量高,经纯化后电泳检测带型清晰.RAPD扩增显示,用该快速、简便、有效的方法提取的基因组DNA能扩增出清晰易辨的带型.
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| 关 键 词： | 冷季型草坪草 基因组DNA提取 RAPD CTAB 冷季型 草坪草 基因组 分别提取 方法 比较 Turfgrass DNA Extraction Methods 快速 显示 RAPD 检测带 电泳 纯化 质量 产率 分离 最佳 结果 |
| 文章编号： | 1673-1409(2005)02-0028-03 |
Comparison of Methods of Genomic DNA Extraction from Cool-season Turfgrass |
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| QIU Yong-fu,TIAN Zhi-hong,YANG Xiao-song.Comparison of Methods of Genomic DNA Extraction from Cool-season Turfgrass[J].Journal of Yangtze University,2005,2(2):28-30,38. |
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| Authors: | QIU Yong-fu TIAN Zhi-hong YANG Xiao-song |
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| Abstract: | The genomic DNA of three kinds of cool-season turfgrass (tall fescue,kentucky bluegrass and perennial ryegrass) was isolated by SDS method,hot CTAB method,high-salt-low-pH method and modified CTAB method. The results indicated that the modified CTAB method was the most effective. High yield ratio,high purity and good quality of the genomic DNA were acquired by the modified CTAB method.Clear DNA electrophoresis bands of purified DNA were displayed. RAPD amplification showed that clear and distinct DNA bands were amplified from the genomic DNA isolated by the modified CTAB method,which is a rapid,simple and effective method. |
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| Keywords: | cool-season turfgrass genomic DNA extraction RAPD CTAB |
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