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Genomic features of Pseudomonas sp. RGM2144 correlates with increased survival of rainbow trout infected by Flavobacterium psychrophilum |
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| Authors: | José M Vidal Alexis Fonseca Pamela Ruiz Daniela Sepúlveda Carlos Carrasco Stefano Scilipoti Javier Barros Ariel Valenzuela Ricardo Saavedra Nathaly Ruiz-Tagle Homero Urrutia |
| Institution: | 1. Laboratorio de Biopelículas y Microbiología Ambiental, Centro de Biotecnología, Universidad de Concepción, Concepción, Chile
Departamento de Investigación y Desarrollo, Ecombio limitada, Concepción, Chile;2. Laboratorio de Biopelículas y Microbiología Ambiental, Centro de Biotecnología, Universidad de Concepción, Concepción, Chile Laboratorio de Bentos, Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción, Chile Center for Electromicrobiology, Department of Biology, Aarhus University, Denmark;3. Laboratorio de Biopelículas y Microbiología Ambiental, Centro de Biotecnología, Universidad de Concepción, Concepción, Chile;4. Independent Researcher, Aarhus, Denmark;5. Departamento de Investigación y Desarrollo, Concepción, Chile;6. Laboratorio de Piscicultura y Patología Acuática, Departamento de Oceanografía, Facultad de Ciencias Naturales y Oceanográficas, Universidad de Concepción, Concepción, Chile |
| Abstract: | This study evaluated the probiotic potential of the biofilm formed by the strain Pseudomonas sp. RGM2144 on rainbow trout survival. When challenged with the fish pathogen Flavobacterium psychrophilum, Pseudomonas sp. RGM2144 increased rainbow trout survival to 92.7 ± 1.2% (control: 35.3 ± 9.5%, p < .0001). The draft genome of Pseudomonas sp. RGM2144 is 6.8 Mbp long, with a completeness 100% and a contamination of 0.4%. The genome contains 6122 protein-coding genes of which 3564 (~60%) have known functions. The genome and phylogeny indicate that Pseudomonas sp. RGM2144 is a new species in the Pseudomonas genus, with few virulence factors, plasmids, and genes associated with antimicrobial resistance, suggesting a non-pathogenic bacterium with protective potential. In addition, the genome encodes for 11 secondary metabolite biosynthetic gene clusters that could be involved in the inhibition of F. psychrophilum. We suggest that Pseudomonas sp. RGM2144 may be applied as a probiotic in salmonid fish farming. |
| Keywords: | Antarctic bacteria Flavobacterium psychrophilum genome probiotic Pseudomonas |