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爬山虎多糖的提取与含量测定
引用本文:夏秋红,杨玲. 爬山虎多糖的提取与含量测定[J]. 塔里木大学学报, 2012, 24(1): 21-24
作者姓名:夏秋红  杨玲
作者单位:1. 塔里木大学生命科学学院,新疆阿拉尔,843300
2. 塔里木大学生命科学学院,新疆阿拉尔843300;新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室,新疆阿拉尔843300
基金项目:新疆生产建设兵团塔里木盆地生物资源保护利用重点实验室开放课题
摘    要:建立了石油醚脱脂—70%乙醇除杂—热水提取爬山虎中多糖的工艺,用苯酚—硫酸法测定多糖的含量。实验表明,此方法精密度、稳定性、重现性较好,回收率为98.07%,RSD=3.72%;熟果、茎和叶的多糖平均含量分别为:6.39%、21.88%、11.77%。

关 键 词:爬山虎  多糖  含量测定

Extracting and Measuring Content of Polysaccharide from Parthenocissus tricuspidata
Xia Qiuhong , Yang Ling. Extracting and Measuring Content of Polysaccharide from Parthenocissus tricuspidata[J]. Journal of tarim University, 2012, 24(1): 21-24
Authors:Xia Qiuhong    Yang Ling
Affiliation:1,2(1 College of Life Science,Tarim University,Alar,Xinjiang 843300)(2 Xinjiang Production &Construction Corps Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin,Alar,Xinjiang 843300)
Abstract:Establishment the extraction process: Petroleum ether De-fatting-70% ethanol refluxing-water extracting,and polysaccharide was extracted from Parthenocissus tricuspidata,phenol-sulfuric acid method was employed to measure polysaccharide content.The experment shows that the method is good in precision,stability and reproducibility,the recovery rate reaches 98.07%,RSD is 3.72%,polysaccharide content of ripe fruits,stem and leave reach 6.39%,21.88% and 11.77%,respectively.
Keywords:Parthenocissus tricuspidata  polysaccharide  content measuring
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