| 龙眼果实ACS和ACO基因克隆及其表达特性 |
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| 引用本文： | 徐士娟,邝健飞,陆旺金,万嗣宝,陈建业. 龙眼果实ACS和ACO基因克隆及其表达特性[J]. 果树学报, 2010, 0(1) |
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| 作者姓名： | 徐士娟 邝健飞 陆旺金 万嗣宝 陈建业 |
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| 作者单位： | 华南农业大学园艺学院广东省果蔬保鲜重点实验室;中国科学院华南植物园;上海大学生命科学学院; |
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| 基金项目： | 广东省自然科学基金团队项目(资助号:06200670) |
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| 摘 要： | 采用RT-PCR和RACE扩增技术相结合的方法从龙眼果实中分离得到ACS和ACO基因,并分别命名为DlACS1和DlACO1。DlACS1蛋白一共编码486个氨基酸,含有7个保守区域和11个不变的氨基残基;DlACO1蛋白一共编码315个氨基酸,含有7个保守区域和9个不变的氨基残基。在龙眼果实发育过程中,果实乙烯释放量在幼果期出现一个小峰,随后逐渐降低,至发育后期维持在较低水平。Northern分析表明,果皮中DlACS1和DlACO1的表达均在果实快速生长期达到最高;在果肉中,DlACS1只在果肉生长初期表达,DlACO1表达随着果肉的生长缓慢降低,在果实成熟期略有升高。此外,DlACS1和DlACO1表达不具有组织特异性,但在各组织中的表达有明显差异。上述结果为研究非跃变型果实的成熟与乙烯之间的关系奠定了基础。
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| 关 键 词： | 龙眼果实 ACS基因 ACO基因 克隆 表达特性 |
Isolation and expression pattern of ACS and ACO genes in longan fruit |
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| XU Shi-juan,KUANG Jian-fei,LU Wang-jin,WAN Si-bao,CHEN Jian-ye. Isolation and expression pattern of ACS and ACO genes in longan fruit[J]. Journal of Fruit Science, 2010, 0(1) |
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| Authors: | XU Shi-juan KUANG Jian-fei LU Wang-jin WAN Si-bao CHEN Jian-ye |
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| Affiliation: | 1Guangdong Key Laboratory for Postharvest Science;College of Horticultural Science;South China Agricultural University;Guangzhou;Guangdong 510642 China;2South China Botanical Garden;Chinese Academy of Sciences;Guangdong 510650 China;3College of Life Sciences;Shanghai University;Shanghai 200444 China |
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| Abstract: | In the present study,ACS and ACO genes from longan termed DlACS1 and DlACO1,were isolated using the combination of RT-PCR and RACE. DlACS1 protein was a polypeptide of 486 amino acids,including 7 conserved regions and 11 conserved amino residues,and DlACO1 protein consisted of 315 amino acids,covering 7 conserved regions and 9 conserved amino residues. Ethylene production exhibited a small ethylene peak at the early stage of fruit development,but decreased slowly thereafter and maintained a very low level a... |
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| Keywords: | Dimocarpus longan ACS gene ACO gene Cloning Expression |
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