不同优势抗虫棉杂交组合不同生育期基因表达差异初探

采用DDRT-PCR技术对3个产量优势差异较大的杂交棉组合及其亲本在4个生育期叶片cDNA进行扩增和差显，结果表明，产量高、中、低优势组合与其亲本基因差异表达比例从蕾期到花铃期总体上呈递减趋势；杂种特异表达在前3个生育期高优势组合高于中或低优势组合，特异表达可能对杂种优势的产生起一定作用；单亲表达一致是基因差异表达中最主要表达模式，但在各生育期高、中、低优势组合之间变化幅度较小，表明此模式可能与杂种优势发挥无明显关系。

Primary Study on Gene Differential Expression of Pest-Resistant Cotton Hybrids between Different Heterosis Crosses

Leaf cDNA of 3 pest-resistant cotton crosses with remarkable different yield heterosis and their parents in 4 growth stages were amplified by DDRT-PCR(mRNA Differential Display) with 45 primers combinations.The results were as follows: The proportions of genes differential expression between F1 and their parents were degressive tendency from square stage to boll stage in these crosses.The percentages of genes differential expression in the high heterotic cross were higher than those in the low heterotic crosses in four growth stages,which showing a closely relationship between gene differential expression and heterosis occurrence.The percentages of M3(bands expressed only in F1) in the high heterotic crosses surpassed those in middle or low heterotic crosses at three earlier growing stages,which suggested M3 played an important role in yield heterosis occurrence.M4(bands expressed in one parent and F1) was the main expression pattern in gene differential expression,but varied within narrow range in four growing stages,which indicated no remarkable correlation between yield heterosis and M4.