| 固定化生姜蛋白酶的制备及其性质研究 |
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| 引用本文： | 崔松松,张琛,何孔泉,张洁.固定化生姜蛋白酶的制备及其性质研究[J].中国饲料,2011(18). |
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| 作者姓名： | 崔松松 张琛 何孔泉 张洁 |
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| 作者单位： | 安徽农业大学生命科学学院 |
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| 基金项目： | 安徽省高校省级科研项目(KJ2010B338); 安徽农业大学SRF项目(09183) |
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| 摘 要： | 本试验以海藻酸钠为载体、戊二醛为交联剂,制备固定化生姜蛋白酶,对固定化条件进行优化并对固定化酶与游离酶的部分酶学性质进行研究。结果表明,固定化生姜蛋白酶的最佳条件是海藻酸钠浓度为2%,CaCl2的浓度为2%,戊二醛的浓度为3%,在此条件下制备的固定化酶活力为53.48 U/g。固定化酶的最适温度是60℃,最适pH值为6.0,与游离酶相比没有发生改变,但固定化酶与游离酶相比对温度和酸碱适应范围更加宽泛。
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| 关 键 词： | 海藻酸钠 戊二醛 固定化 生姜蛋白酶 |
Study on preparation and enzymatic characteristic of immobilized ginger protease |
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| Cui Songsong,Zhang Chen,He Kongquan,et al..Study on preparation and enzymatic characteristic of immobilized ginger protease[J].China Feed,2011(18). |
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| Authors: | Cui Songsong Zhang Chen He Kongquan |
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| Institution: | Cui Songsong,Zhang Chen,He Kongquan,et al. |
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| Abstract: | Ginger proteas was immobilized by encapsulate of sodium alginate and those two were crosslinked with glutaraldehyde.The immobilition conditions and partial properties of immobilized ginger protease and free enzyme were de-termined.The results showed that the optimum operation conditions were:sodium alginate concentration was 2 %,CaCl 2 so-lution concentration was 2 % and glutaradehyde concentration was 3 %.Under this optimum conditions,the activity of in-soluble enzyme was 53.48 U/g.Compared with the free e... |
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| Keywords: | sodium alginate glutaraldehyde immobilization ginger protease |
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