| 苹果多聚半乳糖醛酸酶抑制蛋白的分离纯化 |
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| 引用本文： | 王晓利,张军科,汪勇,杜敬. 苹果多聚半乳糖醛酸酶抑制蛋白的分离纯化[J]. 西北农业学报, 2008, 17(6): 94-97 |
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| 作者姓名： | 王晓利 张军科 汪勇 杜敬 |
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| 作者单位： | 1. 西北农林科技大学,园艺学院,陕西杨凌,712100
2. 西北农林科技大学,资源环境学院,陕西杨凌,712100 |
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| 基金项目： | 国家自然科学基金 |
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| 摘 要： | 以膨大期的富士苹果果皮为材料,通过匀浆、硫酸铵沉淀、透析、聚乙二醇6000浓缩、CM-SephadexC-50离子交换柱和Sephadex G-100分子筛凝胶过滤层析分离纯化出苹果多聚半乳糖醛酸酶抑制蛋白。用还原糖法测定其具有抑制多聚半乳糖醛酸酶活性,bradford法进行定量,经非连续SDS-PAGE分析为电泳纯,根据标准蛋白鉴定其亚基的分子量为41 kD。为苹果PGIP的纯化建立了一种有效的方法。
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| 关 键 词： | 苹果 纯化 多聚半乳糖醛酸酶抑制蛋白 |
| 收稿时间： | 2008-04-09 |
| 修稿时间： | 2008-05-28 |
Isolation and Purification of a Polygalacturonase-inhibiting Protein(PGIP) from Apple |
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| WANG Xiao-li,ZHANG Jun-ke,WANG Yong and DU Jing. Isolation and Purification of a Polygalacturonase-inhibiting Protein(PGIP) from Apple[J]. Acta Agriculturae Boreali-occidentalis Sinica, 2008, 17(6): 94-97 |
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| Authors: | WANG Xiao-li ZHANG Jun-ke WANG Yong DU Jing |
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| Affiliation: | Department of Horticulture, Northwest A&F University, Yangling Shaanxi 712100, China;Department of Horticulture, Northwest A&F University, Yangling Shaanxi 712100, China;Department of Resources and Environment, Northwest A&F University, Yangling Shaanxi 712100, China;Department of Horticulture, Northwest A&F University, Yangling Shaanxi 712100, China |
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| Abstract: | Polygalacturonase-inhibiting protein(PGIP) was purified from extracts of apple cortical tissue by ammonium sulfate precipetation,ion-exchange chrom atography and gel filtration.PGIP activity was messured by determing reducing groups using the dinitrosalicylic acid procedure and PGIP weight was determined by bradford.The purified PGIP showed a single protein band during SDS-PAGE.The apple PGIP subnit has a molecular weight(as deternined by SDSPAGE) about 41kD.This study presented an effective approach for PGIP purification in Malus demestica and provided an experimental basis for the comparative study. |
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| Keywords: | Apple Purification PGIP |
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