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| 农杆菌介导大豆胚尖转化体系的研究 | |
| 引用本文： | 郑丽红,季静,王罡,关春峰,王伟,王萍. 农杆菌介导大豆胚尖转化体系的研究[J]. 作物杂志, 2012, 0(2): 76-79 |
| 作者姓名： | 郑丽红 季静 王罡 关春峰 王伟 王萍 |
| 作者单位： | 1. 天津大学化工学院,300072,天津;天津大学遗传工程研究所,300072,天津 2. 天津大学遗传工程研究所,300072,天津 3. 淮海工学院海洋学院,222005,江苏连云港 |
| 基金项目： | 转基因生物新品种培育重大专项(2008ZX08004-001);国家转基因生物新品种培育重大专项(2009ZX08010-013B) |
| 摘 要： | 以大豆"黑农37"胚尖为外植体,利用农杆菌介导法将抗除草剂基因EPSPS转入大豆,并对转化各培养阶段6-苄氨基腺嘌呤(6-BA)浓度进行了优化。结果表明:在预培养和共培养培养基中加入较高浓度6-BA有利于抗性芽的诱导;1mg/L6-BA和0.2mg/L吲哚丁酸(IBA)配合使用有利于抗性芽的伸长且提高了抗性芽的再生率;将抗性芽添加在1mg/L IBA的培养基中培养7d后转入不加任何激素的培养基中生根快且移栽后成活率高。PCR结果初步证明将EPSPS基因转入到大豆中。 |
| 关 键 词： | 大豆 胚尖 农杆菌介导 6-BA 转化体系 |
Optimization of Agrobacterium-mediated Transformation System of Soybean Embryonic Tip |
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| Zheng Lihong , Ji Jing , Wang Gang , Guan Chunfeng , Wang Wei , Wang Ping. Optimization of Agrobacterium-mediated Transformation System of Soybean Embryonic Tip[J]. Crops, 2012, 0(2): 76-79 | |
| Authors: | Zheng Lihong Ji Jing Wang Gang Guan Chunfeng Wang Wei Wang Ping |
| Affiliation: | 1School of Chemical Engineering and Technology,Tianjin University,300072,Tianjin;2Tianjin Research Institute of Genetic Engineering,300072,Tianjin;3School of Marine Science and Technology,Huaihai Institute of Technology,Lianyungang 222005,Jiangsu,China) |
| Abstract: | The herbicide resistant gene(EPSPS)was transferred into soybean embryonic tip using the Agrobacterium-mediated transformation,and the transformation system was optimized.The results showed that higher 6-BA concentrations in the pre-culture and co-culture medium could induce resistant shoots.The results also indicated that 1mg/L 6-BA and 0.2mg/L IBA could improve regeneration rate of the resistant shoots and elongate resistant shoots length.The shoots rooted quickly when cultured in medium with 1mg/L IBA for 7 days and then transferred to the medium without any hormones.PCR results indicated that the EPSPS gene had been transferred into soybean. |
| Keywords: | Soybean Embryonic tip Agrobacterium-mediated 6-BA Transformation system |
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