| 响应面法优化连翘叶中连翘苷提取工艺研究 |
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| 引用本文： | 王杰,韩晓彤,张薇,李霞. 响应面法优化连翘叶中连翘苷提取工艺研究[J]. 作物研究, 2012, 26(5): 518-522 |
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| 作者姓名： | 王杰 韩晓彤 张薇 李霞 |
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| 作者单位： | 湖南农业大学理学院,长沙,410128 |
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| 基金项目： | 湖南农业大学青年基金(11QN13) |
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| 摘 要： | 通过单因素试验研究乙醇浓度、料液比、提取温度、提取时间和提取次数5因素对连翘叶中连翘苷得率的影响.在单因素试验的基础上,采用四因素三水平的Box - Behnken Design (BBD)中心试验设计研究响应值以及最佳变量的组合,得出连翘叶中连翘苷提取的二次回归方程.通过响应面法综合评价提取连翘叶中连翘苷的最佳条件为80％乙醇、料液比1∶14、83℃、2h,在此条件下的连翘苷得率为0.3895％.
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| 关 键 词： | 连翘 连翘苷 提取工艺 响应面法 |
Study on Extraction Technology of Phillyrin from Forsythia suspensa Leaves using Response Surface Method |
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| WANG Jie , HAN Xiao-tong , ZHANG Wei , LI Xia. Study on Extraction Technology of Phillyrin from Forsythia suspensa Leaves using Response Surface Method[J]. Crop Research, 2012, 26(5): 518-522 |
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| Authors: | WANG Jie HAN Xiao-tong ZHANG Wei LI Xia |
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| Affiliation: | *(College of Science,Hunan Agricultural University,Changsha,Hunan 410128,China) |
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| Abstract: | To obtain the optimal technology for extraction of forsythin from Leaves of Forsythia suspensa,the effects of ethanol concentration,material-to-liquid ratio,extraction temperature,extraction time and extraction times on phillyrin yield were investigated by single factor method.And then a 4-factor,3-level Box-Behnken experimental design coupled with response surface method was employed to optimize conditions.The optimal values of ethanol concentration,material-to-liquid ratio,extraction temperature and extraction time were determined to be 80%,1∶ 14,83℃ and 2 h,respectively.Under this condition,the yield of phillyrin was 0.3895%. |
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| Keywords: | Forsythia suspensa Phillyrin Extraction technology Response surface method |
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