云岭黑山羊BMPR-IB基因部分编码区的克隆及多态性分析

应用RT-PCR方法从云岭黑山羊卵巢组织克隆与产羔性状相关的BMPR-IB基因。结果表明,克隆的BMPR-IB基因扩增片段长467 bp,扩增片段位于该基因编码区第497与963位碱基之间,与已报道的野生型山羊、绵羊、猪、人和鼠的BMPR-IB基因该编码区的同源性分别为99%、99%、92%、92%和87%;与野生型山羊相比,云岭黑山羊BMPR-IB基因第498位和575位碱基存在多态性位点,其中第498位碱基由T突变为C,组成的密码仍编码天冬氨酸（Asp,D）,为同义突变;第575位碱基由C突变为T,编码的氨基酸由脯氨酸（Pro,P）变为亮氨酸（Leu,L）,为错义突变;BMPR-IB蛋白的二级结构在错义突变位点可能具有多种构象。本研究结果为深入研究BMPR-IB基因与云岭黑山羊产羔性状间的关系奠定了基础。

Molecular Cloning and Polymorphism Analysis of the Partial Coding Region of BMPR-IB Gene in Yunling Black Goat

The BMPR-IB gene,which is related to prolific traits of animal,was cloned by RT-PCR from the ovary tissue of Yunling Black goat.The results showed that the amplified sequence of the BMPR-IB gene was 467 bp and it lied between the 497(th) base and the 963(th) base in the coding region of BMPR-IB.The homology of the amplified sequence with the wild goat,sheep,pig,human and rat was 99%,99%,92%,92% and 87%,respectively, in the same coding region of the BMPR-IB gene.Compared to the wild goat,there were two polymorphic sites at the 498(th) and the 575(th) base of the nucleus acid sequence of the amplified region of BMPR-IB gene in Yunling Black goat,which was that the T base mutated to C base at the 498(th) site and coding the same amino acid(Asp),the C base mutated to T base at the 575(th) site and the coding amino acid changed from proline to leucine,and which might lead to the different secondary structure of BMPR-IB protein.These results would be the foundation for further researching the association between BMPR-IB gene and prolific traits of Yunling Black goat.