过氧化物酶同工酶与棉花黄萎病抗性的相关研究

对20个来源及抗性不同的海岛棉和陆地棉品种和2个陆地棉(S)/海岛棉(R)杂交组合 的亲本、 F1、 F2世代的POX同工酶研究表明， 接种黄萎病菌前后， 棉花叶片中的 POX同工酶酶谱发生明显变化， 抗病品种与感病品种的阴性POX同工酶谱带均由原来的3 条 增 至6条， 但两者之间不存在明显差异； 阳性POX同工酶谱带接种前后均只有1条(

Relationship between Peroxidase Isozymes and Resistance to Verticil-lium Wilt in Cotton

Peroxidase(POX)isozyme patterns were investigated in the materials including P1,P2，F1 and F2 of two crosses between susceptible upland cotton cultivars and a resistant seaIsIand cotton variety，and 20 cottons belonging to G．barbadenseand G．hirsutum with differentlevels of Verticillium wilt resistance．There were three POX isozyme bands of anode in both the susceptible and resistant cottons when no pathogen stress，and after inoculation，three new bands of anode appeared in all the materials．On the other hand，one band d cathode，named POXPCl，was different between the susceptible and the resistant materials before inoculation，and the band Of the susceptble varieties was more intensive than that of the resistant. After inoculation，the PCl band of the resistant was still weak，but the band of the susceptible was much stronger than that of uninoculated plants．Throngh the analyses to the PCl band of F1 and F2populations，it was shown that almost all the bands of F1 plants were wenk，and the segrion ratios of wenk and strong bands in F2 populatioas were 3：1．Siace the resistance of the G．barbadense varietywas controlled by a single gene，the PCl band gene with the characteristic of single gene could beregulated or controlled by the resistant gene．In the F2 generation of two combinatious，it was Observed that there was a Significant relatioaship between intensiveness of PCl band and Verticillium wilt resistance, with the performance of light-colored band plant showing resistantand deep-colored band plants susceptible. Therefore, the POX-PCl isozyme can be used as agenetic marker applicable to the future cotton breeding.