大枣多糖铁的合成及其铁含量测定

[目的]从大枣中提取大枣多糖,然后与铁（Ⅲ）合成大枣多糖铁配合物（JPC）,建立JPC中铁含量的测定方法。[方法]采用热水浸泡法提取大枣多糖;在碱性条件下,大枣多糖水溶液与三氯化铁反应合成JPC;采用邻菲罗啉分光光度法和原子吸收光谱法测定JPC中铁（Ⅲ）的含量。[结果]JPC是深棕红色无定型粉末,JPC中铁（Ⅲ）的含量：邻菲罗啉分光光度法测定结果为19.76%,原子吸收光谱法测定结果为20.37%。[结论]邻菲罗啉分光光度法和原子吸收光谱法的测定结果基本一致。

Synthesis of Jujube Polysaccharide-Iron Complex and Determination of Fe Content

[Objective]To prepare jujube polysaccharide-iron(Ⅲ) complex(JPC) by the synthesis of polysaccharide extracted from jujube and FeCl3,and construct a method for determining the Fe content in JPC.[Method]The polysaccharide was extracted from jujube by hot water immersion method,and then synthesized with FeCl3 to produce JPC under alkaline condition;at last the Fe(Ⅲ) content in JPC was detected by phenanthroline spectrophotometry and atomic absorption spectrophometry.[Result]JPC was heavy brownish red and shapeless powder.The content of Fe(Ⅲ) in JPC was 19.76% detected by phenanthroline spectrophotometry and 20.37% detected by atomic absorption spectrophometry.[Conclusion]The results detected by these two methods were basically consistent.