| HPLC-ELSD法测定洒金桃叶珊瑚叶中桃叶珊瑚苷含量的研究 |
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| 引用本文： | 郑敬,魏屹,李静,黄兴悦,陈志远.HPLC-ELSD法测定洒金桃叶珊瑚叶中桃叶珊瑚苷含量的研究[J].安徽农业科学,2011,39(15):8918-8919. |
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| 作者姓名： | 郑敬 魏屹 李静 黄兴悦 陈志远 |
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| 作者单位： | 西南交通大学生命科学与工程学院,四川成都,610031 |
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| 基金项目： | 四川省科技厅科技支撑项目(2008SZ0009) |
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| 摘 要： | 目的]建立HPLC-ELSD测定洒金桃叶珊瑚叶中桃叶珊瑚苷含量的方法。方法]采用蒸发光散射检测器（ELSD）对洒金桃叶珊瑚叶中桃叶珊瑚苷进行了HPLC分析。结果]桃叶珊瑚苷在0.504～2.268μg范围内线性关系良好,回归方程为Y=1.656 4X＋4.434 1（r=0.999 6,n=8）,洒金桃叶珊瑚叶中桃叶珊瑚苷含量为2.64%,平均回收率为99.01%,RSD为1.53%。结论]该方法简便、快速、准确性高、重复性好,适合于洒金桃叶珊瑚叶中桃叶珊瑚苷的定量分析。
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| 关 键 词： | 洒金桃叶珊瑚 桃叶珊瑚苷 HPLC-ELSD 含量测定 |
Determination of Aucubin in the Leaves of Aucuba japonica var.variegata by HPLC-ELSD |
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| ZHENG Jing et al.Determination of Aucubin in the Leaves of Aucuba japonica var.variegata by HPLC-ELSD[J].Journal of Anhui Agricultural Sciences,2011,39(15):8918-8919. |
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| Authors: | ZHENG Jing |
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| Institution: | ZHENG Jing et al(School of Life Science and Engineering,Southwest Jiaotong University,Chengdu,Sichuan 610031) |
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| Abstract: | Objective] The aim was to determine the content of aucubin in the leaves of Aucuba japonica var.variegata with HPLC-ELSD method.Method] The ELSD was adopted to make a HPLC-ELSD analysis for aucubin in the leaves of Aucuba japonica var.variegata.Result] Aucubin showed a good linearity in the range of 0.504-2.268 μg.The regression equation was Y=1.656 4X+4.434 1(r=0.999 6).The content of aucubin in the leaves of Aucuba japonica var.variegata was 2.64 %,the average recovery of aucubin was 99.01 %,and RSD was 1.53%.Conclusion] The method was simple and rapid with reproducibility for the determination of aucubin in the leaves of Aucuba japonica var.variegata. |
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| Keywords: | Aucuba japonica var variegata Aucubin HPLC-ELSD Determination |
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