| ISSR-PCR分子标记法鉴别宁杞1号与雄性不育枸杞 |
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| 引用本文： | 思彬彬,王镇.ISSR-PCR分子标记法鉴别宁杞1号与雄性不育枸杞[J].安徽农业科学,2011,39(14):8309-8310. |
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| 作者姓名： | 思彬彬 王镇 |
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| 作者单位： | 北方民族大学生物科学与工程学院,宁夏银川,750021;北方民族大学生物科学与工程学院,宁夏银川,750021 |
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| 基金项目： | 北方民族大学资助项目(2007Y049) |
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| 摘 要： | 目的]探索在核酸分子水平上鉴别道地药材宁夏枸杞中不同的品种。方法]从50条ISSR引物中筛选合适的引物对宁杞1号与雄性不育2个品种进行PCR扩增及电泳分析,寻找特征位点。结果]1条ISSR引物扩增出较为明显的多态性特征条带,可用于宁杞1号与雄性不育的鉴别。结论]ISSR-PCR作为一种简便、可靠的分子标记方法,可用于枸杞的鉴别。
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| 关 键 词： | 枸杞(Lycium barbarum L.) ISSR 鉴别 |
Identification of No.1 and Male Sterility of Lycium barbarum L.by ISSR-PCR Molecular Marker Technique |
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| SI Bin-bin et al.Identification of No.1 and Male Sterility of Lycium barbarum L.by ISSR-PCR Molecular Marker Technique[J].Journal of Anhui Agricultural Sciences,2011,39(14):8309-8310. |
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| Authors: | SI Bin-bin |
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| Institution: | SI Bin-bin et al(College of Bioscience and Engineering,Northern University for Nationalities,Yinchuan,Ningxia 750021) |
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| Abstract: | Objective]To identify genuine medicinal materials of Lycium barbarum L.by ISSR-PCR molecular marker technique on the level of DNA molecular.Method] Suitable primers were screened out from 50 ISSR primers(Inter-simple sequence repeat),and then used in PCR amplification and electrophoretic analysis for distinguishing Lycium barbarum L.from No.1 and male sterility by finding specific sites.Result]One ISSR primer could amplify polymorphic bands that were suitable for distinguishing Lycium barbarum L.from No.... |
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| Keywords: | Lycium barbarum L ISSR Identification |
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