| 人源明胶基因在毕赤酵母中的表达 |
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| 引用本文： | 段慧明,陈劲春. 人源明胶基因在毕赤酵母中的表达[J]. 安徽农业科学, 2011, 39(16): 9520-9521 |
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| 作者姓名： | 段慧明 陈劲春 |
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| 作者单位： | 北京化工大学生命科学与技术学院,北京,100029;北京化工大学生命科学与技术学院,北京,100029 |
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| 摘 要： | [目的]利用毕赤酵母重组获得明胶蛋白。[方法]构建基因工程菌,利用甲醇诱导表达。[结果]LC-MS/MS检测到了明胶蛋白。[结论]微生物重组表达特定分子量大小的明胶是可行的。
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| 关 键 词： | 毕赤酵母 明胶蛋白 重组表达 |
Expression of Human-derived Gelatin Protein in Pichia pastoris |
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| DUAN Hui-ming et al. Expression of Human-derived Gelatin Protein in Pichia pastoris[J]. Journal of Anhui Agricultural Sciences, 2011, 39(16): 9520-9521 |
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| Authors: | DUAN Hui-ming et al |
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| Affiliation: | DUAN Hui-ming et al(College of Life Science and Technology,Beijing University of Chemical Technology,Beijing 100029) |
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| Abstract: | [Objective] To obtain recombinant gelatin protein by Pichia pastoris.[Method]The genetic engineering bacteria was constructed,and induced by methanol.[Result] The gelatin protein was detected by LC-MS/MS.[Conclusion] The expression of specific molecular weight of gelatin by recombinant microorganism was feasible. |
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| Keywords: | Pichia pastoris Gelatin protein Recombinant expression |
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