大豆细菌性斑点病菌hrpZPsg12基因增强病原菌对大豆的致病性并引起烟草过敏性反应

目的]明确hrpZPsg12基因对大豆细菌性斑点病菌致病性的影响。方法]采用PCR方法从大豆细菌性斑点病菌中克隆hrpZPsg12基因,利用具有自杀特性的敲除质粒pKNOCK-Cm和具有功能互补作用的粘粒pUFR034,构建了hrpZPsg12基因的突变载体pKNOCK477-7和互补载体pUFR1026-68,并筛选出hrpZPsg12基因的突变体477-1及其功能互补子1026-5。进一步将野生型Psg12、突变体477-1和互补子反应斑。但是,反应斑的大小有差异,Psg12菌株接种的病斑较大,477-1的较小,互补子1026-5的接近野生型菌株Psg12。病斑中细菌繁殖量分析表明,野生型菌株Psg12繁殖量最高,突变体477-1的最低,互补子1026-5的接近野生型菌株Psg12的繁殖量。结论]hrpZ-Psg12基因能够增强大豆细菌性斑点病菌对大豆的致病性,并且能够在烟草上产生过敏性反应。

Enhancement of the Pathogenicity of Pathogen on Soybean and Cause of Tobacco Hypersensitive Response by hrpZPsg12 Gene of Pseudomonas syringae pv.Glycinea

Objective] The paper was to confirm the effect of hrpZPsg12 gene on the pathogenicity of Pseudomonas syringae pv.glycinea.Method] hrpZPsg12 gene was cloned from P.syringae pv.glycinea using PCR method.The knockout plasmid pKNOCK-Cm with suicide characteristics and clay pUFR034 with complementarity were used to construct the mutation vector pKNOCK477-7 and complementary vector pUFR1026-68 of hrpZPsg12 gene,the mutant 477-1 and the functional complementation unit 1026-5 of the gene was also screened out.Thr...