| 环介导等温扩增技术快速检测志贺氏菌的研究 |
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| 引用本文： | 马晓燕,张会彦,宋明明,张先舟,王羽,张伟.环介导等温扩增技术快速检测志贺氏菌的研究[J].安徽农业科学,2011,39(14):8191-8193,8240. |
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| 作者姓名： | 马晓燕 张会彦 宋明明 张先舟 王羽 张伟 |
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| 作者单位： | 河北农业大学食品科技学院,河北保定,071001;河北农业大学食品科技学院,河北保定,071001;河北农业大学食品科技学院,河北保定,071001;河北农业大学食品科技学院,河北保定,071001;河北农业大学食品科技学院,河北保定,071001;河北农业大学食品科技学院,河北保定,071001 |
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| 基金项目： | 河北省自然科学基金项目(C2008000216) |
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| 摘 要： | 目的]利用环介导等温扩增技术快速检测志贺氏菌。方法]以志贺氏菌侵袭性质粒抗原H基因（ipaH）作为靶序列,设计引物,优化Mg2＋浓度、Bst酶浓度等反应条件,建立LAMP反应体系。结果]确定了环介导等温扩增技术检测志贺氏菌的适宜反应条件;该法检测志贺氏菌的灵敏度为62 cfu/ml。结论]该研究初步建立了一种利用LAMP快速检测志贺氏菌的方法,为食品中志贺氏菌快速检测构建了一个技术平台。
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| 关 键 词： | 环介导等温扩增技术 检测 志贺氏菌 |
Research on the Detection of Shigella by LAMP |
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| MA Xiao-yan et al.Research on the Detection of Shigella by LAMP[J].Journal of Anhui Agricultural Sciences,2011,39(14):8191-8193,8240. |
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| Authors: | MA Xiao-yan |
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| Institution: | MA Xiao-yan et al(Colloge of Food Science & Technology,Hebei Agriculture University,Baoding,Hebei 071001) |
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| Abstract: | Objective] The aim was to detect Shigella by using the loop-mediated isothermal amplification(LAMP) technology.Method] The sequences of ipaH of Shigella was used as target sequences to design primers,the reaction conditions such as Mg2+ concentration,Bst DNA polymerase concentration were optimized for establishing LAMP system.Result] The reaction conditions were optimized and the sensitivity of LAMP assay was 62 cfu/ml.Conclusion] LAMP technology for detection of Shigella was developed,and a technology ... |
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| Keywords: | LAMP Detection Shigella |
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