| 微针注射甜玉米胚芽gus基因转化瞬时表达研究 |
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| 引用本文： | 刘立岩. 微针注射甜玉米胚芽gus基因转化瞬时表达研究[J]. 安徽农业科学, 2011, 39(15): 8883-8884,8886 |
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| 作者姓名： | 刘立岩 |
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| 作者单位： | 铁岭师范高等专科学校,辽宁铁岭,112000 |
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| 摘 要： | [目的]研究微针注射甜玉米（Zea mays L.）胚芽gus基因转化瞬时表达,为玉米遗传转化的进一步研究奠定基础。[方法]以农杆菌为介导,采用gus基因瞬时表达技术,建立了胚芽注射开放性基因转化体系。[结果]3 mm长的胚芽为最适转化受体;农杆菌EHA105菌液浓度最适OD值为0.558～0.630;gus基因转化最适共培养天数为3 d。在以上最适转化条件下,微针注射胚芽生长点基因转化率为1%。[结论]该研究可为改良甜玉米遗传性状,培育基因工程新品种提供参考。
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| 关 键 词： | 甜玉米 微针 注射 胚芽 基因转化 瞬时表达 |
Research of gus Gene Transformation Transient Expression on Microneedle Injection Sweet Corn(Z.mays L.) Germ |
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| LIU Li-yan. Research of gus Gene Transformation Transient Expression on Microneedle Injection Sweet Corn(Z.mays L.) Germ[J]. Journal of Anhui Agricultural Sciences, 2011, 39(15): 8883-8884,8886 |
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| Authors: | LIU Li-yan |
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| Affiliation: | LIU Li-yan(Tieling Normal College,Tieling,Liaoning 112000) |
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| Abstract: | [Objective] The aim to study the gus gene transformation transient expression on microneedle injection sweet corn germ,to lay basis for deeply researching corn transformation.[Method] The experiment was done by using gus gene transient expression technology mediated by agrobacterium,and a embryo injection system for open gene transformation was built.[Result] The results showed that the seeds germ with the length of 3 mm was the most optimal transformation receptor.The optimal OD value of EHA105 agrobacteri... |
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| Keywords: | Sweet corn Microneedle Injection Germ Gene transformation Transient expression |
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