| 氧化铝-活性炭吸附法纯化透明质酸的条件优化 |
| |
| 引用本文： | 刘晓连,罗瑞明,佘永红,柳杨.氧化铝-活性炭吸附法纯化透明质酸的条件优化[J].安徽农业科学,2011,39(15):8832-8835. |
| |
| 作者姓名： | 刘晓连 罗瑞明 佘永红 柳杨 |
| |
| 作者单位： | 宁夏大学农学院,宁夏银川,750021;宁夏大学农学院,宁夏银川,750021;宁夏大学农学院,宁夏银川,750021;宁夏大学农学院,宁夏银川,750021 |
| |
| 基金项目： | 宁夏回族自治区科技攻关计划项目(NXGG2009-4) |
| |
| 摘 要： | 目的]研究用氧化铝-活性炭吸附法去除透明质酸（HA）中热原的优化条件,为生产高安全性的HA提供参考。方法]通过分别对氧化铝添加量、第1次搅拌时间、活性炭添加量和第2次搅拌时间进行单因素试验和二次回归正交组合试验,以HA回收率和热原的含量作为评定指标,对氧化铝-活性炭吸附法纯化HA的条件进行了优化。结果]优化后的条件：氧化铝添加量为80.00 g/L,第1次搅拌时间为1.00 h,活性炭添加量为5.00 g/L,第2次搅拌时间为2.00 h,此时的HA回收率最高,热原含量合格。结论]对于发酵法制备的HA,用氧化铝和活性炭吸附的方法来去除热原比较简便,且处理后的HA热原含量合格。
|
| 关 键 词： | 氧化铝 活性炭 搅拌时间 透明质酸 热原 |
Condition Optimization for Purifying Hyaluronic Acid with Method of Alumina and Activated Carbon Absorption |
| |
| LIU Xiao-lian et al.Condition Optimization for Purifying Hyaluronic Acid with Method of Alumina and Activated Carbon Absorption[J].Journal of Anhui Agricultural Sciences,2011,39(15):8832-8835. |
| |
| Authors: | LIU Xiao-lian |
| |
| Institution: | LIU Xiao-lian et al(Agricultural College of Ningxia University,Yinchuan,Ningxia 750021) |
| |
| Abstract: | Objective] The study aimed to research the optimized condition for eliminating the pyrogen in the hyaluronic acid(HA)with method of alumina and activated carbon absorption.Method] With the HA recovery and pyrogen content in HA as the evaluation index,the conditions for purifying HA with the method of alumina and activated carbon absorption were optimized through the single factor test and the square recurrence orthogonal test resp.with the 4 factors of Alumia dosage,first stirring time,activated carbon do... |
| |
| Keywords: | Alumina Activated carbon Stiring time Hyaluronic acid Pyrogen |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
