| 响应曲面法优化苏云金芽孢杆菌B28培养基 |
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| 引用本文： | 刘坤,郭威,胡雪芹,张洪斌. 响应曲面法优化苏云金芽孢杆菌B28培养基[J]. 安徽农业科学, 2011, 39(16): 9690-9692,9694 |
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| 作者姓名： | 刘坤 郭威 胡雪芹 张洪斌 |
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| 作者单位： | 合肥工业大学化工学院,安徽,合肥,230009 |
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| 基金项目： | 国家大学生创新性实验计划(gdcx200941) |
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| 摘 要： | [目的]优化苏云金芽孢杆菌B28培养基,提高晶体蛋白产量。[方法]采用SAS软件设计PB试验,得到影响晶体蛋白含量的显著因素。根据选出的显著因素设计最陡爬坡试验,确定晶体蛋白含量最高的中心点。根据确定的显著因素和中心点设计响应曲面法分析试验。[结果]最佳培养基配比：牛肉膏4.00 g/L,蛋白胨10.00 g/L,葡萄糖3.00 g/L,NaCl 4.60 g/L,K2HPO40.67 g/L,MgSO40.30g/L,MnSO40.05 g/L。[结论]优化后的B28晶体蛋白产量提升了43.5%。
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| 关 键 词： | 响应曲面法 苏云金芽孢杆菌 培养基优化 晶体蛋白 |
Bacillus thuringiensis B28 Medium Optimized by the Response Surface Methodology |
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| LIU Kun et al. Bacillus thuringiensis B28 Medium Optimized by the Response Surface Methodology[J]. Journal of Anhui Agricultural Sciences, 2011, 39(16): 9690-9692,9694 |
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| Authors: | LIU Kun et al |
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| Affiliation: | LIU Kun et al (College of Chemical Engineering,Hefei University of Technology,Hefei,Anhui 230009) |
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| Abstract: | [Objective] The research aimed to optimize Bacillus thuringiensis B28 medium to improve the crystal protein yield.[Method] By using SAS software,PB test was designed to obtain the significant factors which affected the crystal protein content.According to the selected significant factors,the steepest ascent experiment was designed to determine the central point where the crystal protein content was highest.Based on the determined significant factors and center point,the response surface methodology analysis... |
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| Keywords: | Response surface methodology Bacillus thuringiensis Medium optimization Crystal protein |
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