牛杀菌／通透性增加蛋白氮端基因的克隆和序列分析

本试验应用RT-PCR技术，参照Genbank报道的序列，从荷斯坦牛中性粒细胞mRNA中扩增出杀菌／通透性增加蛋白（BPI）氮端基因（713bp），并与pGEM-T-easy载体连接，构建基因重组体pGEM-T-easy-BPI，进行序列测定。结果表明获得长度为713bp的BPI氮端基因。序列分析证实该片段与安哥斯牛BPI氮端基因相比有1个点突变，为同义突变。该基因的克隆为进一步表达该基因奠定了基础。

Cloning and Sequence Analyzing of Bovine Bactericidal/permeability-increasing Protein N-terminal Fragment

The study aimed to clone cDNA of N-terminal bovine bactericidal/permeability-increasing protein（BPI）and to construct the recombinant plasmid pGEM-T-easy-BPL According to the sequence in Genbank, the gene which encodes N-terminal BPI protein was amplified by RT-PCR from mRNA that were extracted from the bovine polymorphonuclear neutrophils （PMN）.The PCR product was cloned into the pGEM-T-easy vector and the sequence was confirmed by sequencing. The result showed that a 713bp gene was acquired. Compared with the sequence in Genbank, there is a silent mutation of one base pair.